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- Clone
- OX-18 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MHC Class I, RT1.A
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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LOU rat splenocytes stained with OX-18 PE
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 205208 | 100 µg | $223 | ||||
The rat monomorphic MHC class I antigen RT1A is expressed on nucleated cells (except neurons and glial cells) and erythrocytes. RT1A is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins.
Product DetailsProduct Details
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- RT1B-depleted membrane glycoproteins isolated from Wistar rat spleen
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (For the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, immnoprecipitation, and blocking function
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Application References
(PubMed link indicates BioLegend citation) -
- Hellstrom M, et al. 2014. Acta Biomater. 10:5034. PubMed
- Product Citations
-
- RRID
-
AB_1186083 (BioLegend Cat. No. 205208)
Antigen Details
- Distribution
- Nucleated cells and erythrocytes
- Biology Area
- Immunology
- Molecular Family
- MHC Antigens
- Gene ID
- 100188935 View all products for this Gene ID
- UniProt
- View information about RT1A on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All RT1A Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-rat RT1A | OX-18 | FC,IHC-F,IP |
| PE anti-rat RT1A | OX-18 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-rat RT1A
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PE anti-rat RT1A
LOU rat splenocytes stained with OX-18 PE
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