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PE anti-TCF1 (TCF7) Antibody

Pricing & Availability
Clone
7F11A10 (See other available formats)
Regulatory Status
RUO
Other Names
T-cell-specific transcription factor 1, transcription factor 7, TCF7
Isotype
Mouse IgG1, κ
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Product Citations
publications
7F11A10_PE_TCF1_Antibody_022818
Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with TCF1 (TCF7) (clone 7F11A10) PE (left) or mouse IgG1, κ PE isotype control (right).
  • 7F11A10_PE_TCF1_Antibody_022818
    Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with TCF1 (TCF7) (clone 7F11A10) PE (left) or mouse IgG1, κ PE isotype control (right).
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655207 25 tests $141
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655208 100 tests $360
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Description

TCF1 is the first identified member of the T-cell-specific transcription factor family.  It plays an important role in T cell development and differentiation. TCF1 is inactivated by association with the transcriptional repressor TLE proteins. During Wnt signaling, the transcriptional coactivator CTNNB1 accumulates and, in turn, replaces the transcriptional repressor associated with TCF1. Interaction with CTNNB1 results in transactivation of TCF1 target genes. Deletion of TCF1 causes massive apoptosis of double positive thymocyte, suggesting that TCF1 is required for thymocyte survival during T cell development. In addition to its function in thymus, TCF1 promotes T cell differentiation to Th2 cells in the periphery through transcriptional activation of GATA3.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial TCF1 recombinant protein (116-334 aa)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per 106 cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Ye L, et al. 2022. Cell Metab. 34:595. PubMed
  2. Li X, et al. 2023. J Clin Invest. 133:. PubMed
  3. Ando M, et al. 2021. Cancer Res Commun. 1:41. PubMed
  4. Au L, et al. 2021. Cancer Cell. 39:1497. PubMed
  5. Braun DA, et al. 2021. Cancer Cell. 39:632. PubMed
  6. Mold JE, et al. 2021. Cell Reports. 35(8):109174. PubMed
  7. Collins PL et al. 2018. Cell. 176(1-2):348-360 . PubMed
  8. Liang T, et al. 2022. Front Immunol. 13:985280. PubMed
RRID
AB_2728491 (BioLegend Cat. No. 655207)
AB_2728491 (BioLegend Cat. No. 655208)

Antigen Details

Structure
384 amino acids, predicted molecular weight of 42 kD. Contains a HMG box DNA binding domain and a CTNNB1 (β-catenin) binding domain.
Distribution

Nucleus

Function
TCF1 is a transcription factor, involved in the canonical Wingless/Integration 1 (Wnt) signaling pathway. TCF1 is essential for survival of CD4+CD8+ double positive thymocytes and differentiation of T cells in the periphery.
Interaction
TCF1 interacts with CTNNB1, TLE1, TLE2, TLE3, TLE4, and AES.
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers, TCRs
Antigen References

1. Mao CD, et al. 2011. Crit. Rev. Eukaryot. Gene Expr. 21:207.
2. Germar K, et al. 2011. P. Natl. Acad. Sci. USA 108:20060.
3. Wang R, et al. 2011. J. Immunol. 187:5964.
4. Weber BN, et al. 2011. Nature 476:63.
5. Jeannet G, et al. 2010. P. Natl. Acad. Sci. USA 107:9777.
6. Staal FJ, et al. 1999. Int. Immunol. 11:317.

Gene ID
6932 View all products for this Gene ID
UniProt
View information about TCF1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 03/01/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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