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PE/Cyanine5 anti-mouse CD127 (IL-7Rα) Antibody

Pricing & Availability
Clone
SB/199 (See other available formats)
Regulatory Status
RUO
Other Names
IL-7 receptor α chain, IL-7Rα
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
SBslash199_PEcyaine5_CD127_Antibody_112923
C57BL/6 splenocytes stained with mouse CD127 (IL-7Ra) (clone SB/199) PE/Cyanine5 and CD3 APC (left) or rat IgG2b, κ PE/Cyanine5 isotype control (right).
  • SBslash199_PEcyaine5_CD127_Antibody_112923
    C57BL/6 splenocytes stained with mouse CD127 (IL-7Ra) (clone SB/199) PE/Cyanine5 and CD3 APC (left) or rat IgG2b, κ PE/Cyanine5 isotype control (right).
Compare all formats See PE/Cyanine5 spectral data
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121123 25 µg $129
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121124 100 µg $340
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Description

CD127 is a 60-90 kD type I transmembrane glycoprotein, also known as IL-7 receptor α chain or IL-7Rα. It forms heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse pre-B cell line 1A9
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine5 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining.

Application References

(PubMed link indicates BioLegend citation)
  1. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
  2. Kouro T, et al. 2002. Blood 100:3672.
  3. Maeda K, et al. 2005. Blood 106:879.
  4. Diao J, et al. 2004. J. Immunol. 173:1826.
RRID
AB_2832431 (BioLegend Cat. No. 121123)
AB_2832431 (BioLegend Cat. No. 121124)

Antigen Details

Structure
Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Distribution

Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells

Function
T cell and immature B cell proliferation and development
Ligand/Receptor
IL-7
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Sudo T, et al. 1993. Proc. Natl. Acad. Sci. USA. 90:9125.
2. He YW and Malek TR. 1998. Crit Rev. Immunol. 18:503.
3. Huster K M,et al. 2004. Proc. Natl. Acad. Sci. USA. 101:5610.
4. Pillai M, et al. 2004. Leuk Lymphoma. 45:2403.
5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.

Gene ID
16197 View all products for this Gene ID
UniProt
View information about CD127 on UniProt.org
Go To Top Version: 1    Revision Date: 12/09/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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