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PE/Cyanine5 anti-rat CD161 Antibody

Pricing & Availability
Clone
3.2.3 (See other available formats)
Regulatory Status
RUO
Other Names
NKR-P1, CD161a/CD161b, NKR-P1a/KLRB1a
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
3dot2dot3_PECy5_CD161_Antibody_081921.png
Rat splenocytes were stained with anti-rat CD3 PE and CD161 (clone 3.2.3) PE/Cyanine5 or mouse IgG1, κ PE/Cyanine5 isotype control (right).
  • 3dot2dot3_PECy5_CD161_Antibody_081921.png
    Rat splenocytes were stained with anti-rat CD3 PE and CD161 (clone 3.2.3) PE/Cyanine5 or mouse IgG1, κ PE/Cyanine5 isotype control (right).
Compare all formats See PE/Cyanine5 spectral data
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205611 25 µg $101
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205612 100 µg $288
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Description

CD161 is a 30 kD type II transmembrane C-type lectin, expressed as a homodimer. Rat NKR-P1 receptors are primarily expressed on NK cells, a subset of T cells, dendritic cells, and activated monocytes. There are three different types of NKR-P in rat, namely NKR-P1a, NKR-P1b, and NKR-P1c. NKR-P1a does not contain an ITIM structure and is an activating receptor, while NKR-P1b contains an ITIM and displays inhibitory function. LLT-1 (ligand lectin like transcript 1) is the ligand, while KLR (killer cell lectin like) functions as a receptor.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
LEW rat splenic NK cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine5 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

3.2.3 recognizes a common epitope of NKR-1P1a (CD161a) and NKR-P1b (CD161b).

Application References

(PubMed link indicates BioLegend citation)
  1. Chambers W, et al. 1989. J. Exp. Med. 169:1373. (FC)
RRID
AB_2894438 (BioLegend Cat. No. 205611)
AB_2894438 (BioLegend Cat. No. 205612)

Antigen Details

Structure
Type II transmembrane C-type lectin, 30 kD.
Distribution

NK cells, subset of T cells, dendritic cells, activated monocytes.

Function
NKR-P1a is a NK cell-activating receptor; NKR-P1b is a NK cell-inhibitory receptor.
Ligand/Receptor
LLT1-ligand lectin like transcript 1; KLR-killer cell lectin like receptor.
Cell Type
Dendritic cells, Monocytes, NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Chambers WH, et al. 1989. J. Exp. Med. 169:1373.
2. Brink M, et al. 1990. J. Exp. Med. 171:197.
3. Li J, et al. 2003. Int. Immunol. 15:411.

Gene ID
362443 View all products for this Gene ID
UniProt
View information about CD161 on UniProt.org
Go To Top Version: 1    Revision Date: 08/19/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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