PE/Cyanine7 anti-β Catenin 1 Antibody, beta Catenin 1, 15B8

Pricing & Availability

Clone: 15B8 (See other available formats)
Regulatory Status: RUO
Other Names: β-catenin, CTNNB1
Isotype: Mouse IgG1, κ
Ave. Rating: Submit a Review
Product Citations: publications

15B8_PECyanine7_betaCatenin1_Antibody_060922 — Human colon adenocarcinoma cell line SW480 were treated with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and stained with anti-β Catenin 1 (clone 15B8) PE/Cyanine7 (filled histogram) or mouse IgG1, κ PE/Cyanine (open historgram).

Compare all formats See PE/Cyanine7 spectral data

Cat #

Size

Price

Quantity Check Availability

Save

862605

25 tests

$206

Check Availability

Need larger quantities of this item?
Request Bulk Quote

862606

100 tests

$429

Check Availability

Need larger quantities of this item?
Request Bulk Quote

Description

β catenin is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. β catenin also plays a key role in Wnt signaling pathways and thus is involved in neural differentiation, synaptic plasticity, neurodegenerative disease, and prevention of apoptosis. This protein also binds to adenomatous polyposis coli, which is mutated in the adenomatous polyposis of the colon.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2927830 (BioLegend Cat. No. 862605)
AB_2927830 (BioLegend Cat. No. 862606)

Antigen Details

Structure: β Catenin is a 781 amino acid protein with an apparent molecular mass of ~ 86 kD.
Distribution: Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Plasma membrane, cytoskeleton, nucleus, cytosol, and extracellular
Function: β Catenin is a dual functional protein involved in cell-cell adhesion and gene transcription.
Interaction: E-cadherin/CDH1, LEF1, TCF4, etc.
Antigen References: Dias C, et al. 2014. Nature. 516(7529): 51

Rosiles A, et al. 2016. CNS Neurol Disord Drug Targets. 15(1):3

Na K, et al. 2017. Anticancer Res. 37(6):3249

Zhu P, et al. 2018. Cancer Res Treat. 50(4):1452
Gene ID: 1499 View all products for this Gene ID 12387 View all products for this Gene ID 84353 View all products for this Gene ID
UniProt: View information about beta Catenin 1 on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Intracellular Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Related FAQs

Other Formats

View All β Catenin 1 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-β Catenin 1	15B8	WB,IHC-P,ICFC
PE anti-β Catenin 1	15B8	ICFC
PE/Cyanine7 anti-β Catenin 1	15B8	ICFC

Certificate of Analysis

Customers Also Purchased

PE anti-mouse TCR Vβ11

Clone: RR3-15

MojoSort™ Mouse CD4 T Cell Isolation Kit

Brilliant Violet 421™ anti-mouse/human CD324 (E-Cadherin)

Clone: DECMA-1

Brilliant Violet 711™ anti-mouse CD366 (Tim-3)

Clone: RMT3-23

Go To Top Version: 1 Revision Date: 06/09/2022

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.