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PE/Cyanine7 anti-mouse CD23 Antibody

Pricing & Availability
Clone
B3B4 (See other available formats)
Regulatory Status
RUO
Other Names
FcεRII, IgE Fc Receptor, BLAST-2, Ly-42, B6
Isotype
Rat IgG2a, κ
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Product Citations
publications
B3B4_PECy7_080207
CD57BL/6 mouse splenocytes were stained with CD23 (clone B3B4) PE/Cyanine7 (filled histogram), or rat IgG2a, κ PE/Cyanine7 (open histogram).
  • B3B4_PECy7_080207
    CD57BL/6 mouse splenocytes were stained with CD23 (clone B3B4) PE/Cyanine7 (filled histogram), or rat IgG2a, κ PE/Cyanine7 (open histogram).
Compare all formats See PE/Cyanine7 spectral data
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101613 25 µg $106
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101614 100 µg $216
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Description

CD23 is a 45 kD protein also known as low affinity IgE Fc receptor, FcεRII, BLAST-2, Ly-42, or B6. It is a member of the Ig family, expressed on conventional B (but not B-1) cells and follicular dendritic cells. CD23 responds to high levels of IgE by downregulating IgE secretion.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Complex of IgE with Fcε receptor isolated from the mouse B hybridoma cell line O1.2B2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The B3B4 antibody is useful for blocking IgE activity in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy, in vitro and in vivo blocking of ligand binding2-4, immunohistochemical staining of acetone-fixed frozen sections5, and spatial biology (IBEX)7,8.

Application References

(PubMed link indicates BioLegend citation)
  1. Waldschmidt TJ, et al. 1988. J. Immunol. 140:2148. (IP)
  2. Rao M, et al. 1987. J. Immunol. 138:1845. (Block)
  3. Oshiba A, et al. 1997. J. Immunol. 159:4056. (Block)
  4. Dasic G, et al. 1999. Eur. J. Immunol. 29:2957. (Block)
  5. Maeda K, et al. 1992. J. Immunol. 148:2340. (IHC)
  6. Craig VJ, et al. 2011. Cancer Res. 71:3616. PubMed
  7. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  8. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Meyer SN, et al. 2020. Immunity. 51(3):535-547. PubMed
  2. Masle-Farquhar E, et al. 2023. Front Immunol. 14:1095257. PubMed
  3. Gómez-Díaz C, et al. 2021. iScience. 24:103241. PubMed
  4. Holodick N, et al. 2014. J Immunol. 192:2432. PubMed
  5. Onodera T, et al. 2016. J Immunol. 196: 4172 - 4184. PubMed
  6. Hutter K, et al. 2020. FEBS J. . PubMed
  7. Sangesland M, et al. 2020. Cell Reports. 32(8):108065. PubMed
  8. Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
  9. Chiu Y, et al. 2014. J Immunol. 193:2207. PubMed
  10. Holodick N, et al. 2016. J Immunol. 196: 4348 - 4357. PubMed
  11. Wang YH, et al. 2019. Front Immunol. 10:1909. PubMed
  12. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  13. Riedel R, et al. 2020. Nat Commun. 11:2570. PubMed
  14. Kimura S, et al. 2020. Am J Transplant. 20:977. PubMed
  15. Vijay R, et al. 2020. Nat Immunol. 21:790. PubMed
  16. Qiu Z, et al. 2020. Cancer Genet. 245:6. PubMed
  17. Fiala G, et al. 2015. J Exp Med. 212: 1693-1708. PubMed
  18. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
RRID
AB_2103036 (BioLegend Cat. No. 101613)
AB_2103036 (BioLegend Cat. No. 101614)

Antigen Details

Structure
C-lectin family, type II transmembrane glycoprotein, 45 kD
Distribution

B cells, follicular dendritic cells

Function
Regulates B cell activation
Ligand/Receptor
IgE, CD19/CD21/CD81
Cell Type
B cells, Dendritic cells
Biology Area
Immunology
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Delespesse G, et al. 1992. Immunol. Rev. 125:77.
3. Flores-Romo L, et al. 1993. Science 261:1038.

Gene ID
14128 View all products for this Gene ID
UniProt
View information about CD23 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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