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PE/Cyanine7 anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
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Product Citations
publications
104_PECy7_021009.jpg
C57BL/6 mouse splenocytes stained with 104 PE/Cyanine7
  • 104_PECy7_021009.jpg
    C57BL/6 mouse splenocytes stained with 104 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
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109829 25 µg $66
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109830 100 µg $209
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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3. 

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed
  2. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  3. He D, et al. 2022. Immunity. 55:159. PubMed
  4. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  5. Simpson AP, et al. 2022. Cell Rep. 40:111099. PubMed
  6. Frederico B, et al. 2022. Dev Cell. 57:1957. PubMed
  7. Zhao M, et al. 2022. Nat Commun. 13:7600. PubMed
  8. Giannou AD, et al. 2023. Immunity. 56:125. PubMed
  9. Oh S, et al. 2023. Nat Biotechnol. :. PubMed
  10. Ernst MPT, et al. 2023. Hemasphere. 7:e824. PubMed
  11. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  12. Wang YC, et al. 2023. J Exp Med. 220:. PubMed
  13. Cheng MI, et al. 2023. Nat Immunol. 24:780. PubMed
  14. Kurosawa M, et al. 2021. Int J Mol Sci. 22:. PubMed
  15. Koh CH, et al. 2020. Cancer Immunol Res. 8:698. PubMed
  16. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
  17. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  18. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  19. Mylonas K, et al. 2015. Immunobiology. 220: 924-933. PubMed
  20. Christian LS, et al. 2021. Cell Reports. 35(6):109118. PubMed
  21. Roeschert I, et al. 2021. Nat Cancer. 2:312. PubMed
  22. Bangs DJ, et al. 2022. Cell Rep. 38:110266. PubMed
  23. Kremenovic M, et al. 2022. J Immunother Cancer. 10:. PubMed
  24. Kumaraswami K, et al. 2020. Int J Mol Sci. 21:00. PubMed
  25. Gruber T, et al. 2020. JCI Insight. 5:00. PubMed
  26. Li H, Watowich S 2014. J Vis Exp. 85: 51189. PubMed
  27. Schwartz DM et al. 2019. Immunity. 50(1):106-120 . PubMed
  28. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  29. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  30. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  31. Casali P, et al. 2021. Front Immunol. 12:761450. PubMed
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  33. LaMothe RA et al. 2018. Front Immunol. 0.570138889 . PubMed
  34. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  35. Tang Y, et al. 2015. Exp Hematol. 43: 554-564. PubMed
  36. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  37. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  38. Kim P, et al. 2015. J Exp Med. 212:633. PubMed
  39. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
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  42. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  43. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  44. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  45. Moutuou MM, et al. 2020. Methods Mol Biol. 2111:267. PubMed
  46. Bastow CR, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  47. Daneshmandi S, et al. 2021. Elife. 10:. PubMed
  48. Steinbicker A, et al. 2011. Blood. 117:4915. PubMed
  49. Beura LK, et al. 2018. Immunity. 48:327. PubMed
  50. Suan D et al. 2017. Immunity. 47(6):1142-1153 . PubMed
  51. Tuong ZK, et al. 2021. iScience. 24:103326. PubMed
  52. Nuro-Gyina PK, et al. 2021. J Immunol. 207:868. PubMed
  53. Anderson-Baucum E, et al. 2021. Cell Metab. 33:1883. PubMed
  54. Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed
  55. Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed
  56. van den Berk P, et al. 2020. Cell Rep. 33:108533. PubMed
  57. Rodríguez L, et al. 2021. Biomolecules. 11: . PubMed
  58. Sheng J, et al. 2021. eLife. 10:00. PubMed
  59. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  60. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  61. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  62. Zhang B, et al. 2021. Nature. 599:471. PubMed
  63. Spath S, et al. 2022. iScience. 25:104998. PubMed
  64. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  65. Deets KA, et al. 2021. Elife. 10:. PubMed
  66. Sun D, et al. 2020. PLoS Pathog. 16:e1008361. PubMed
  67. Koronyo Y, et al. 2015. Brain. 138: 2399-2422. PubMed
  68. Puleston D, et al. 2014. Elife. 3:3706. PubMed
  69. Muri J, et al. 2020. Cell Reports. 30(13):4399-4417. PubMed
  70. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
  71. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
RRID
AB_1186103 (BioLegend Cat. No. 109829)
AB_1186103 (BioLegend Cat. No. 109830)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45.2 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse CD45.2 104 FC
FITC anti-mouse CD45.2 104 FC
PE anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 104 FC,Block,IHC-F,IP
APC anti-mouse CD45.2 104 FC
Alexa Fluor® 488 anti-mouse CD45.2 104 FC
Alexa Fluor® 647 anti-mouse CD45.2 104 FC
Pacific Blue™ anti-mouse CD45.2 104 FC
Alexa Fluor® 700 anti-mouse CD45.2 104 FC
APC/Cyanine7 anti-mouse CD45.2 104 FC
PerCP anti-mouse CD45.2 104 FC
PerCP/Cyanine5.5 anti-mouse CD45.2 104 FC
PE/Cyanine7 anti-mouse CD45.2 104 FC
Brilliant Violet 421™ anti-mouse CD45.2 104 FC
Brilliant Violet 570™ anti-mouse CD45.2 104 FC
Brilliant Violet 650™ anti-mouse CD45.2 104 FC
Brilliant Violet 510™ anti-mouse CD45.2 104 FC
Brilliant Violet 785™ anti-mouse CD45.2 104 FC
Brilliant Violet 605™ anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 (Maxpar® Ready) 104 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.2 104 FC
Brilliant Violet 711™ anti-mouse CD45.2 104 FC
Alexa Fluor® 594 anti-mouse CD45.2 104 IHC-F,SB
APC/Fire™ 750 anti-mouse CD45.2 104 FC
TotalSeq™-A0157 anti-mouse CD45.2 104 PG
TotalSeq™-B0157 anti-mouse CD45.2 104 PG
TotalSeq™-C0157 anti-mouse CD45.2 104 PG
Brilliant Violet 750™ anti-mouse CD45.2 104 FC
Spark Blue™ 550 anti-mouse CD45.2 104 FC
Spark NIR™ 685 anti-mouse CD45.2 104 FC
Spark UV™ 387 anti-mouse CD45.2 104 FC
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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