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PE/Cyanine7 anti-mouse CD64 (FcγRI) Antibody

Pricing & Availability
Clone
X54-5/7.1 (See other available formats)
Regulatory Status
RUO
Other Names
FcRI
Isotype
Mouse IgG1, κ
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Product Citations
publications
X545-71_PECyanine7_CD64_Antibody_1_030624
C57BL/6 mouse bone marrow cells were stained with CD11b APC and CD64 (clone X54-5/7.1) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right). Data shown was gated on myeloid cell population.
  • X545-71_PECyanine7_CD64_Antibody_1_030624
    C57BL/6 mouse bone marrow cells were stained with CD11b APC and CD64 (clone X54-5/7.1) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right). Data shown was gated on myeloid cell population.
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139313 25 µg $176
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139314 100 µg $382
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Description

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BALB/c mouse FcγRI-human IgG Fc fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.

Application References

(PubMed link indicates BioLegend citation)
  1. Tan PS, et al. 2003. J. Immunol. 170:2549. (IP)
  2. Ingersoll MA, et al. 2010. Blood 115:e10. (FC)
  3. Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed
  4. Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Cohen K, et al. 2021. Cell Rep. 37:110026. PubMed
  2. Ozga AJ, et al. 2022. Immunity. 55:82. PubMed
  3. Zhou H, et al. 2022. EMBO Rep. 23:e53791. PubMed
  4. Akter S, et al. 2022. Cell Rep. 39:110983. PubMed
  5. Mugarza E, et al. 2022. Sci Adv. 8:eabm8780. PubMed
  6. Huseni MA, et al. 2023. Cell Rep Med. 4:100878. PubMed
  7. Draijer C, et al. 2023. Front Immunol. 13:1056477. PubMed
  8. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  9. Gallizioli M, et al. 2020. Cell Rep. 33:108291. PubMed
  10. Zhang L, et al. 2022. Immunol Cell Biol. 100:691. PubMed
  11. Bohrer AC, et al. 2022. Cell Rep. 40:111144. PubMed
  12. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  13. Chang CY, et al. 2022. Sci Adv. 8:eabq0615. PubMed
  14. Peng W, et al. 2022. Cell Mol Life Sci. 79:602. PubMed
  15. Zhao Q, et al. 2023. Signal Transduct Target Ther. 8:40. PubMed
  16. Larson-Casey JL, et al. 2023. JCI Insight. 8:. PubMed
  17. Pankhurst TE, et al. 2023. Cell Rep. 42:112310. PubMed
  18. Philpott J, et al. 2022. Immunohorizons. 6:366. PubMed
  19. Larson-Casey JL, et al. 2021. FASEB J. 35:e21675. PubMed
  20. Larsen SE, et al. 2021. Sci Rep. 11:9040. PubMed
  21. Böttcher JP, et al. 2018. Cell. 172:1022. PubMed
  22. Mitsune A, et al. 2021. Respir Res. 22:232. PubMed
  23. Helm M, et al. 2022. Life (Basel). 12:. PubMed
  24. Atkin-Smith G, et al. 2017. Sci Rep. 7:39846. PubMed
  25. Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed
  26. Antunes KH, et al. 2022. Front Immunol. 13:867022. PubMed
  27. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  28. Fantauzzi MF, et al. 2021. ERJ Open Res. 7: . PubMed
  29. Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed
  30. Affo S, et al. 2021. Cancer Cell. 39(6):866-882.e11. PubMed
  31. Smith AS, et al. 2022. J Immunother Cancer. 10:. PubMed
  32. Hole CR, et al. 2019. Nat Commun. 10:2955. PubMed
  33. Freemerman AJ, et al. 2019. J Immunol. 202:1265. PubMed
  34. Alikhanyan K, et al. 2021. Cancers (Basel). 13:. PubMed
  35. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  36. Yadav P, et al. 2021. J Immunol. 206:2888. PubMed
  37. Wu Y, et al. 2021. PLoS Negl Trop Dis. 15:e0010050. PubMed
  38. Liu CY, et al. 2020. Cell Rep. 33:108275. PubMed
  39. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  40. Macdougall CE et al. 2018. Cell metabolism. 27(3):588-601 . PubMed
  41. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
  42. Casulli J, et al. 2019. Nat Commun. 10:2121. PubMed
  43. Maulloo CD, et al. 2021. Front Immunol. 12:714842. PubMed
  44. Larson-Casey JL, et al. 2021. J Biol Chem. :100810. PubMed
  45. Zheng J, et al. 2020. Nature. . PubMed
  46. Olson GS, et al. 2021. Cell Reports. 35(9):109195. PubMed
  47. Kaya B, et al. 2020. Cell Reports. 32(5):107979. PubMed
  48. Smith CA, et al. 2019. JCI Insight. 4. PubMed
  49. Luo J et al. 2018. Immunity. 49(1):107-119 . PubMed
  50. Giampazolias E, et al. 2021. Cell. . PubMed
  51. Murray MP, et al. 2022. Cell Rep. 38:110209. PubMed
  52. Chen F, et al. 2022. Cell Rep. 38:110215. PubMed
  53. Youshani AS, et al. 2019. J Neuroinflammation. 16:25. PubMed
  54. Carlson PM, et al. 2021. J Immunol. 207:720. PubMed
  55. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  56. Xu J, et al. 2020. Immunity. 53(2):371-383.e5. PubMed
  57. Birkl D, et al. 2019. FASEB J. 33:13632. PubMed
  58. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  59. Choi J, et al. 2020. Cell Stem Cell. 27:366. PubMed
  60. Rochford I, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 321:L686. PubMed
  61. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  62. Bosteels C, et al. 2020. Immunity. 52(6):1039-1056.e9.. PubMed
  63. Rappe JCF, et al. 2021. J Exp Med. 218:. PubMed
  64. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  65. Larson–Casey JL, et al. 2019. J Clin Invest. 129:4962. PubMed
  66. Wang L, et al. 2020. J Nanobiotechnology. 18:38. PubMed
RRID
AB_2563903 (BioLegend Cat. No. 139313)
AB_2563903 (BioLegend Cat. No. 139314)

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 72 kD
Distribution

Monocytes, macrophages, mast cells, dendritic cells

Function
Phagocytosis, ADCC
Ligand/Receptor
IgG
Cell Type
Dendritic cells, Macrophages, Mast cells, Monocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Gene ID
14129 View all products for this Gene ID
UniProt
View information about CD64 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 12/14/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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