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PE/Cyanine7 anti-mouse IL-27 p28 Antibody

Pricing & Availability
Clone
MM27-7B1 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-27 p28, IL-27-A, IL-27 subunit alpha
Isotype
Mouse IgG2a
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Product Citations
publications
MM27-7B1_PECyanine7_IL-27_Antibody_1_111424
Thioglycolate-elicited Balb/c peritoneal macrophages primed with IFN-γ for 2 hours, followed by overnight LPS-stimulation, then intracellularly stained with CD107b (Mac-3) (clone M3/84) Alexa Fluor® 647 and IL-27 p28 (clone MM27-7B1) PE/Cyanine7 (left) or mouse IgG2a isotype control (right)
  • MM27-7B1_PECyanine7_IL-27_Antibody_1_111424
    Thioglycolate-elicited Balb/c peritoneal macrophages primed with IFN-γ for 2 hours, followed by overnight LPS-stimulation, then intracellularly stained with CD107b (Mac-3) (clone M3/84) Alexa Fluor® 647 and IL-27 p28 (clone MM27-7B1) PE/Cyanine7 (left) or mouse IgG2a isotype control (right)
Compare all formats See PE/Cyanine7 spectral data
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516910 100 µg $364
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Description

IL-27 is a heterodimeric cytokine consisting of EBV-induced gene-3 (EBI3, an IL-12 p40-related protein) and p28 (a newly discovered IL-12 P35-related protein). It is a member of the IL-6/IL-12 cytokine family and mainly produced by antigen-presenting cells, including macrophages and dendritic cells. IL-27 acts on T cells and NK cells. It has been reported that IL-27 drives rapid clonal expansion of naïve CD4+ T cells, and promotes Th1 polarization and IFN-γ production in synergy with IL-12. The IL-27-induced Th1 differentiation was mediated by rapid and marked upregulation of ICAM-1/LFA-1 interaction in a STAT1-dependent manner. IL-27 exhibits anti-inflammatory function by enhancing Th1 cell differentiation, a potent antitumor activity, through CD8+ T cell and NK cell activation. It also plays a potential therapeutic role in autoimmune disease by inhibiting Th-17 development. IL-27 mediates its biological effects through its receptor, WSX-1/T cell cytokine receptor (TCCR), which is homologous to the IL-12Rβ2 subunit. Protein gp130 serves as a functional signal-transducing molecule for IL-27.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse IL-27-OVA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone MM27-7B1 specifically recognizes the p28 subunit of IL-27.1 We confirmed clone MM27-7B1 cross-reactivity to recombinant human IL-27 in-house using an immunoassay.

ELISA Capture: To measure mouse IL-27, this antibody can be used as a capture antibody in sandwich ELISA format and paired with the biotinylated B02P6E6 antibody (Cat. No. 525904) as the detection antibody. Recombinant Mouse IL-27 (ELISA Std.) (Cat. No. 577409) can be used as the protein standard.
Flow Cytometry: The fluorochrome-labeled MM27-7B1 antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify granulysin-producing cells within mixed cell populations.
Note: For measuring mouse IL-27 in serum, plasma or cell culture supernatant, LEGEND MAX™ Mouse IL-27 Heterodimer ELISA Kit with Pre-coated Plates (Cat. No. 438707 & 438708) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Uyttenhove C, et al. 2011. J. Leuko. Biol. 89:1001.
RRID
AB_10641290 (BioLegend Cat. No. 516910)

Antigen Details

Structure
A heterodimeric cytokine consisting of EBV-induced gene-3 (EBI3, an IL-12 p40-related protein) and p28 (a newly discovered IL-12 p35-related protein).
Distribution

Produced by antigen-presenting cells, including macrophages and dendritic cells.

Function
Drives rapid clonal expansion of naïve CD4+ T cells, promotes Th1 polarization and IFN-γ production; plays a role in the anti-inflammatory and antitumor reponses, and has potential in autoimmune disease therapy.
Interaction
T cells and NK cells.
Ligand/Receptor
IL-27R (TCCR/WSX-1).
Cell Type
Antigen-presenting cells, Dendritic cells, Macrophages
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Salcedo R, et al. 2004. J. Immunol. 173:7170.
2. Owaki T, et al. 2005. J. Immunol. 175:2191.
3. Pflanz S, et al. 2002. Immunity 16:779.
4. Diveu C, et al. 2009. J. Immunol. 182:5748.
5. Morishima N, et al. 2005. J. Immunol. 175:1686.
6. Liu J, et al. 2007. J. Exp. Med. 204:141.

Gene ID
246779 View all products for this Gene ID
UniProt
View information about IL-27 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 09/22/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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