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PE/Cyanine7 anti-mouse TIGIT (Vstm3) Antibody

Pricing & Availability
Clone
1G9 (See other available formats)
Regulatory Status
RUO
Other Names
VSIG9
Isotype
Mouse IgG1, κ
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Product Citations
publications
1G9_PECyanine7_TIGIT_Antibody_1_102623
Con-A stimulated C57BL/6 mouse splenocytes (three days) were stained with CD3 APC and TIGIT (clone 1G9) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right).
  • 1G9_PECyanine7_TIGIT_Antibody_1_102623
    Con-A stimulated C57BL/6 mouse splenocytes (three days) were stained with CD3 APC and TIGIT (clone 1G9) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right).
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142107 25 µg $147
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142108 100 µg $382
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Description

T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as V-set and transmembrane domain-containing protein 3 (Vstm3), is a 26 kD, type I transmembrane protein and member of the CD28 family. TIGIT is expressed on activated T cells, follicular T helper, memory, and regulatory T cells as well as on NK cells. Its binding partners include CD155 (PVR) and CD112 (PVRL2). TIGIT is a negative regulator of NK and T cell activation. Engagement of TIGIT by dendritic cells results in their differentiation into a tolerogenic phenotype, with an increased secretion of IL-10 and a diminished production of IL-12. Mice deficient for TIGIT are more susceptible to autoimmune disease.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Joller N, et al. 2010. J. Immunol. 186:1338.
Product Citations
  1. Bertrand F, et al. 2017. Nat Commun. 8:2256. PubMed
  2. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  3. Weulersse M, et al. 2020. Immunity. 53(4):824-839.e10. PubMed
  4. Zhao H, et al. 2021. Int J Nanomedicine. 16:4913. PubMed
  5. De Ponte Conti B, et al. 2021. Elife. 10:. PubMed
  6. Xiao S, et al. 2020. Cell Reports. 32(2):107892. PubMed
  7. Tian M, et al. 2021. Elife. 10:. PubMed
  8. Zhao H, et al. 2022. Int J Biol Sci. 18:154. PubMed
  9. Barsoumian HB, et al. 2022. Cancers (Basel). 14:. PubMed
  10. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
RRID
AB_2565648 (BioLegend Cat. No. 142107)
AB_2565648 (BioLegend Cat. No. 142108)

Antigen Details

Structure
Type I transmembrane protein, 26 kD, member of the CD28 family. Contains 1 Ig-like domain and 1 cytoplasmic ITIM motif.
Distribution

Activated T cells, follicular T helper cells, NK, memory T cells, Tregs.

Function
Negative regulator of T cell activation and proliferation. Induction of tolerogenic dendritic cells.
Ligand/Receptor
CD155 (PVR) and CD112 (PVRL2).
Cell Type
Dendritic cells, NK cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
Adhesion Molecules, Immune Checkpoint Receptors
Antigen References

1. Levin SD, et al. 2011. Eur. J. Immunol. 41:902.
2. Yu X, et al. 2009. Nat. Immunol. 10:48.
3. Stanietsky N, et al. 2009. P. Natl. Acad. Sci. USA 106:17858.

Gene ID
100043314 View all products for this Gene ID
UniProt
View information about TIGIT on UniProt.org
Go To Top Version: 2    Revision Date: 09/19/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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