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PE/Cyanine7 anti-ZAP70 Phospho (Tyr292) Antibody

Pricing & Availability
Clone
A16038B (See other available formats)
Regulatory Status
RUO
Other Names
p Zeta-Chain (TCR) Associated Protein Kinase 70 kD, p 70 KD Zeta-Chain Associated Protein, p Tyrosine-protein kinase ZAP-70
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A16038B_PE-CYA7_ZAP70_Antibody_032224
Human peripheral blood lymphocytes were treated with (left), or without (right) Hydrogen Peroxide for 5 minutes, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), then surface stained with anti-human CD3 (clone UCHT1) Brilliant Violet 421™ and intracellularly stained with anti-ZAP70 Phospho (Tyr292) (clone A16038B) PE/Cyanine7.
  • A16038B_PE-CYA7_ZAP70_Antibody_032224
    Human peripheral blood lymphocytes were treated with (left), or without (right) Hydrogen Peroxide for 5 minutes, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), then surface stained with anti-human CD3 (clone UCHT1) Brilliant Violet 421™ and intracellularly stained with anti-ZAP70 Phospho (Tyr292) (clone A16038B) PE/Cyanine7.
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693811 25 tests $160
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693812 100 tests $395
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Description

ZAP70 was identified in TCR-stimulated Jurkat cells. It is an inactive cytosolic tyrosine kinase that is recruited to a transmembrane receptor lacking intrinsic catalytic activity. ZAP70 and the related spleen tyrosine kinase (Syk) play a critical role in T-cell development and activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn.

ZAP70 activation can be regulated by binding to phosphorylated ITAMs of the TCR and by phosphorylation of multiple tyrosine residues on ZAP70. Phosphorylation of Tyr315 and Tyr319 are essential for ZAP70 positive regulation of T-lymphocyte activation whereas Tyr292 has a negative regulatory role.

Mutation of Tyr292 to phenylalanine enhanced NFAT induction in response to BCR stimulation, indicating phosphorylation of Tyr292 may promote association of ZAP-70 with a negative regulatory protein. ZAP-70 has been reported to bind Cbl following TCR stimulation and phosphorylation of Tyr292 was required for this interaction, as well as for the negative effect of Cbl on signaling.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human ZAP70 peptide phosphorylated at Tyr292. Complete Freund's adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

During development, reactivity was observed with mouse phospho-SYK.

RRID
AB_3106095 (BioLegend Cat. No. 693811)
AB_3106095 (BioLegend Cat. No. 693812)

Antigen Details

Structure
619 amino acids with a predicted molecular weight of 70 kD.
Distribution

Cytoplasm; Human T Cells, Mouse T cells and B cells.

Function
Required for the assembly of a Zap-70-containing signaling complex. Activation of the PLC-γ1-dependent and Ras-dependent signaling cascades. Associates with PLC-γ 1.
Interaction
NFAM1, Cbl, SLA and SLA2, CBLB, DEF6, FCRL3, VAV1, CD247/CD3Z.
Cell Type
B cells, T cells
Biology Area
Cell Biology, Immunology
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase, TCRs
Antigen References

1. Chan AC, et al. 1991. Proc. Natl. Acad. Sci. USA 88:9166.
2. Arpaia E, et al. 1994. Cell 76:947.
3. Chan AC, et al. 1994. Science 264:1599.
4. Negishi I, et al. 1995. Nature 376:435.
5. Wang H, et al. 2010. Cold Spring Harb. Perspect. Biol. 2:a002279.
6. Klammt C, et al. 2015. Nat. Immunol. 16:961.
7. Weiss A, et al. 1993. Cell 73:209.
8. Gong Q, et al. 2001. J. Exp. Med. 194:507.
9. Magnan A, et al. 2001. J. Exp. Med. 194:491.

Gene ID
7535 View all products for this Gene ID
UniProt
View information about ZAP70 Phospho Tyr292 on UniProt.org
Go To Top Version: 1    Revision Date: 03/11/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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