PE/Fire™ 640 anti-human CD66b Antibody, CD66b, 6/40c

Pricing & Availability

Clone: 6/40c (See other available formats)
Regulatory Status: RUO
Other Names: CEACAM8, CD67, CGM6, NCA-95
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human peripheral blood granulocytes were stained with anti-human CD66b PE/Fire™ 640 (clone 6/40c) (filled histogram) or mouse IgG1, κ isotype control PE/Fire™ 640 (open histogram).

Compare all formats See PE/Fire™ 640 spectral data

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Size

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392917

25 tests

$129

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392918

100 tests

$352

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Description

CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant soluble human CEACAM8-Fc produced in HEK293 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 640 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire™ 640 has a maximum excitation of 566 nm and a maximum emission of 639 nm.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2876709 (BioLegend Cat. No. 392917)
AB_2876709 (BioLegend Cat. No. 392918)

Antigen Details

Structure: Ig superfamily, CEA antigen group, GPI-linked glycoprotein
Distribution: Granulocytes
Function: Cell adhesion, neutrophil activation
Ligand/Receptor: CD66c
Cell Type: Granulocytes
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Kuijpers T, et al. 1993. J Immunol. 151:4934.

Kuroki M, et al. 1992. J Leuk Biol. 52:551.
Gene ID: 1088 View all products for this Gene ID
UniProt: View information about CD66b on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Pathways

Myeloid-Derived Suppressor Cells

Related FAQs

Other Formats

View All CD66b Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD66b	6/40c	FC,IHC-P,SB
PE anti-human CD66b	6/40c	FC
TotalSeq™-A0166 anti-human CD66b	6/40c	PG
Alexa Fluor® 594 anti-human CD66b	6/40c	IHC-P
TotalSeq™-C0166 anti-human CD66b	6/40c	PG
Alexa Fluor® 647 anti-human CD66b	6/40c	IHC-P,FC
TotalSeq™-B0166 anti-human CD66b	6/40c	PG
PE/Fire™ 640 anti-human CD66b Antibody	6/40c	FC
Brilliant Violet 421™ anti-human CD66b Antibody	6/40c	FC,IHC-P
TotalSeq™-D0166 anti-human CD66b	6/40c	PG
TotalSeq™-Bn0166 anti-human CD66b	6/40c	SB
Brilliant Violet 510™ anti-human CD66b	6/40c	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 09/11/2020

For Research Use Only. Not for diagnostic or therapeutic use.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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