PE/Fire™ 640 anti-human CD95 (Fas) Antibody , CD95, DX2

Pricing & Availability

Clone: DX2 (See other available formats)
Regulatory Status: RUO
Workshop: VI C-64
Other Names: Fas, APO-1, TNFRSF6
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human peripheral blood lymphocytes were stained with anti-human CD95 (Fas) (clone DX2) PE/Fire™ 640 (filled histogram), or mouse IgG1, ĸ PE/Fire™ 640 isotype control (open histogram).

Compare all formats See PE/Fire™ 640 spectral data

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305657

25 tests

$147

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305658

100 tests

$347

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Description

CD95 is a 45 kD single chain type I glycoprotein also known as Fas, APO-1, and TNFRSF6. It is a member of the TNF receptor superfamily. CD95 is expressed on T and B lymphocytes, monocytes, neutrophils, and fibroblasts. CD95 expression is upregulated by activation. The extracellular region of CD95 binds to CD178 (Fas ligand). CD178 binding to CD95 induces apoptosis and has been shown to play a role in the maintenance of peripheral tolerance.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Pigtailed Macaque, Sooty Mangabey
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CD95 transfected L cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 640 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire™ 640 has a maximum excitation of 566 nm and a maximum emission of 639 nm.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The DX2 antibody is useful for inducing apoptosis of Fas-positive cells. Additional reported applications (for the relevant formats) include: in vitro induction of apoptosis³ (DX2 antibody is required to be cross-linked for effective induction of apoptosis) and immunohistochemical staining^4,5 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 305655 and 305656).

Note: EOS9.1 antibody can induce apoptosis without cross-linking.
RRID: AB_2892365 (BioLegend Cat. No. 305657)
AB_2892365 (BioLegend Cat. No. 305658)

Antigen Details

Structure: TNFR superfamily, type I transmembrane protein, 45 kD
Distribution: T cells and B cells, monocytes, neutrophils, fibroblasts, expression level upregulated on activated lymphocytes
Function: Mediates apoptosis
Ligand/Receptor: Fas ligand (CD178)
Cell Type: B cells, Fibroblasts, Lymphocytes, Monocytes, Neutrophils, T cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Krammer P, et al. 1994. Immunol. Rev. 142:175.
2. Nagata S, et al. 1995. Science 267:1449.
Gene ID: 355 View all products for this Gene ID
UniProt: View information about CD95 on UniProt.org

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PE/Cyanine7 anti-human CD95 (Fas)	DX2	FC
Brilliant Violet 605™ anti-human CD95 (Fas)	DX2	FC
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Purified anti-human CD95 (Fas) (Maxpar® Ready)	DX2	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD95 (Fas)	DX2	FC
APC/Fire™ 750 anti-human CD95 (Fas)	DX2	FC
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Brilliant Violet 510™ anti-human CD95 (Fas)	DX2	FC
Brilliant Violet 711™ anti-human CD95 (Fas)	DX2	FC
Brilliant Violet 785™ anti-human CD95 (Fas)	DX2	FC
Brilliant Violet 650™ anti-human CD95 (Fas)	DX2	FC
Alexa Fluor® 700 anti-human CD95 (Fas)	DX2	FC
TotalSeq™-A0156 anti-human CD95 (Fas)	DX2	PG
TotalSeq™-C0156 anti-human CD95 (Fas)	DX2	PG
TotalSeq™-B0156 anti-human CD95 (Fas)	DX2	PG
Ultra-LEAF™ Purified anti-human CD95 (Fas)	DX2	FC,ICC,IHC,Apop
TotalSeq™-D0156 anti-human CD95 (Fas)	DX2	PG
PE/Fire™ 640 anti-human CD95 (Fas)	DX2	FC
KIRAVIA Blue 520™ anti-human CD95 (Fas)	DX2	FC
APC/Fire™ 810 anti-human CD95 (Fas) Antibody	DX2	FC
PE anti-human CD95	DX2	FC
Spark YG™ 593 anti-human CD95 (Fas)	DX2	FC
PE/Fire™ 700 anti-human CD95 (Fas)	DX2	FC
PerCP/Fire™ 780 anti-human CD95 (Fas)	DX2	FC
Spark Blue™ 574 anti-human CD95 (Fas)	DX2	FC
Spark Red™ 718 anti-human CD95 (Fas)	DX2	FC
GMP PE anti-human CD95 (Fas)	DX2	FC
PerCP/Fire™ 806 anti-human CD95 (Fas)	DX2	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 06/08/2021

For Research Use Only. Not for diagnostic or therapeutic use.

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