PE/Fire™ 744 anti-human CD152 (CTLA-4) Antibody, CD152, BNI3

Pricing & Availability

Clone: BNI3 (See other available formats)
Regulatory Status: RUO
Other Names: CTLA4, Cytotoxic T-Lymphocyte Antigen 4, CTLA-4
Isotype: Mouse IgG2a, κ
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Product Citations: publications

BNI3_PE-FIRE-744_CD152_Antibody_112323 — Human peripheral blood mononuclear cells were stimulated with Cell Activation Cocktail (without Brefeldin) (Cat. Nos. 423301 and 423302) for 4 hours, surface stained with anti-human CD3 (clone UCHT1) APC. Cells were then fixed, permeabilized, and intracellularly stained with anti-human CD152 (CTLA-4) (clone BNI3) PE/Fire™ 744 (left), or stained with mouse IgG2a, κ PE/Fire™ 744 isotype control (right).

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369645

25 tests

$210

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369646

100 tests

$430

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Description

CD152, also known as Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4), is a 33 kD member of the immunoglobulin superfamily. It is transiently expressed on activated T cells. CTLA-4 is expressed on the surface of helper T cells and transmits an inhibitory signal to T cells. Regulatory T cells express high levels of CTLA-4. CTLA-4 (CD152) is similar to CD28 in amino acid sequence, structure, and genomic organization. Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses through interaction with CD80 (B7-1) and CD86 (B7-2) present on antigen presenting cells (APC). CTLA-4 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.

Mutations in the CTLA-4 gene have been associated with various autoimmune diseases, such as systemic lupus erythematosus, insulin-dependent diabetes mellitus, and other autoimmune diseases. A transcript of the CTLA-4 gene that may represent a native soluble form of CTLA-4 (sCTLA-4) showed that eleven of twenty patients with autoimmune thyroid disease (ATD) had a high concentration of sCTLA-4, whereas only 1 of 30 apparently healthy volunteers contained measurable levels. sCTLA-4 immunoreactivity was inhibited by its binding to B7.1, suggesting that sCTLA-4 is a functional receptor. sCTLA-4 also plays a role in the initial immune response to infection of immune cells by HIV, along with the CD-1 pathway and others.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Extracellular domain of human CTLA-4 and constant regions of the human IgG heavy chain (CTLA-4/IgG)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 744 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
FC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

True-Stain Monocyte Blocker™ (Cat. No. 426102) is recommended to minimize non-specific staining of PE/Fire™ 744 on monocytes and macrophages.

* PE/Fire™ 744 has a maximum excitation of 565 nm and a maximum emission of 744 nm.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Based on in-house testing, we do not recommend using clone BNI3 for immunohistochemistry of paraffin-embedded tissue section.
RRID: AB_3097415 (BioLegend Cat. No. 369645)
AB_3097415 (BioLegend Cat. No. 369646)

Antigen Details

Structure: Ig superfamily and 33 kD.
Distribution: Activated T cells and B cells.
Function: Negative regulator of T cell activation.
Interaction: Antigen presenting cells, such as dendritic cells.
Ligand/Receptor: B7-1 (CD80), B7-2 (CD86).
Cell Type: B cells, T cells
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Kuiper HM, et al. 1995. J. Immunol. 155:1776.
2. Castan J, et al. 1997. Immunology 90:265.
3. Lee CC, et al. 2009. Pediatr. Allergy Immunol. 20:624.
4. Pistillo MP, et al. 2003. Blood 101:202.
5. Tan PH, et al. 2005. Blood. 106:2936.
6. Steiner K, et al. 2001. Clin. Exp. Immunol. 126:143.
Gene ID: 1493 View all products for this Gene ID
UniProt: View information about CD152 on UniProt.org

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Other Formats

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Description	Clone	Applications
Purified anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
PE anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
Brilliant Violet 421™ anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
Brilliant Violet 605™ anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
PerCP/Cyanine5.5 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
Biotin anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
APC anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
PE/Cyanine7 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
PE/Dazzle™ 594 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
TotalSeq™-A0151 anti-human CD152 (CTLA-4)	BNI3	PG
TotalSeq™-C0151 anti-human CD152 (CTLA-4)	BNI3	PG
Brilliant Violet 785™ anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
TotalSeq™-B0151 anti-human CD152 (CTLA-4)	BNI3	PG
APC/Fire™ 750 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
Alexa Fluor® 647 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
APC/Cyanine7 anti-human CD152 (CTLA-4) Antibody	BNI3	ICFC,FC
Brilliant Violet™ 711 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
TotalSeq™-D0151 anti-human CD152 (CTLA-4)	BNI3	PG
PE/Fire™ 640 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
Spark Red™ 718 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
PE/Cyanine5 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
PE/Fire™ 744 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
PE/Fire™ 810 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
Brilliant Violet 650™ anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
Brilliant Violet 510™ anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
Spark Blue™ 574 anti-human CD152 (CTLA-4) (Flexi-Fluor™)	BNI3	FC
Spark Blue™ 550 anti-human CD152 (CTLA-4) (Flexi-Fluor™)	BNI3	FC

Certificate of Analysis

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Recombinant Mouse CTLA-4-Fc Chimera (carrier-free)

Go To Top Version: 1 Revision Date: 11/22/2023

For Research Use Only. Not for diagnostic or therapeutic use.

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