PE/Fire™ 744 anti-human CD4 Antibody, CD4, SK3

Pricing & Availability

Clone: SK3 (See other available formats)
Regulatory Status: RUO
Other Names: T4, Leu3a
Isotype: Mouse IgG1, κ
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Product Citations: publications

SK3_PE-FIRE-744_CD4_Antibody_112223 — Human peripheral blood mononuclear cells were stained with anti-human CD3 (clone UCHT1) KIRAVIA Blue 520™ and anti-human CD4 (clone SK3) PE/Fire™ 744 (left) or mouse IgG1, κ PE/Fire™ 744 isotype control (right).

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344629

25 tests

$130

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344630

100 tests

$340

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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 744 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

True-Stain Monocyte Blocker™ (Cat. No. 426102) is recommended to minimize non-specific staining of PE/Fire™ 744 on monocytes and macrophages.

* PE/Fire™ 744 has a maximum excitation of 565 nm and a maximum emission of 744 nm.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_3097455 (BioLegend Cat. No. 344629)
AB_3097455 (BioLegend Cat. No. 344630)

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution: T cell subset, majority of thymocytes, monocytes/macrophages
Function: MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor: MHC class II molecules, HIV gp120, IL-16
Cell Type: Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Center D et al. 1996. Immunol. Today 17:476.
2. Gaubin M et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.
Gene ID: 920 View all products for this Gene ID
UniProt: View information about CD4 on UniProt.org

Documentation

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Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications
PerCP anti-human CD4	SK3	FC
Purified anti-human CD4	SK3	FC,IP
FITC anti-human CD4	SK3	FC
PE anti-human CD4	SK3	FC
PerCP/Cyanine5.5 anti-human CD4	SK3	FC
Biotin anti-human CD4	SK3	FC
PE/Cyanine7 anti-human CD4	SK3	FC
APC anti-human CD4	SK3	FC
APC/Cyanine7 anti-human CD4	SK3	FC
Alexa Fluor® 488 anti-human CD4	SK3	FC
Pacific Blue™ anti-human CD4	SK3	FC
Alexa Fluor® 700 anti-human CD4	SK3	FC
Purified anti-human CD4 (Maxpar® Ready)	SK3	FC,CyTOF®
PE anti-human CD4	SK3	FC
Brilliant Violet 421™ anti-human CD4	SK3	FC
Brilliant Violet 510™ anti-human CD4	SK3	FC
Alexa Fluor® 647 anti-human CD4	SK3	FC
FITC anti-human CD4	SK3	FC
APC/Fire™ 750 anti-human CD4	SK3	FC
PE/Dazzle™ 594 anti-human CD4	SK3	FC
Pacific Blue™ anti-human CD4	SK3	FC
Brilliant Violet 785™ anti-human CD4	SK3	FC
Brilliant Violet 750™ anti-human CD4	SK3	FC
Brilliant Violet 605™ anti-human CD4	SK3	FC
Brilliant Violet 711™ anti-human CD4	SK3	FC
PE/Cyanine7 anti-human CD4	SK3	FC
TotalSeq™-A0045 anti-human CD4	SK3	PG
TotalSeq™-C0045 anti-human CD4	SK3	PG
PE/Cyanine5 anti-human CD4	SK3	FC
PerCP/Cyanine5.5 anti-human CD4	SK3	FC
Spark Blue™ 550 anti-human CD4	SK3	FC
Spark NIR™ 685 anti-human CD4	SK3	FC
APC anti-human CD4	SK3	FC
KIRAVIA Blue 520™ anti-human CD4	SK3	FC
APC/Fire™ 810 anti-human CD4	SK3	FC
PE/Fire™ 640 anti-human CD4	SK3	FC
PE/Fire™ 700 anti-human CD4	SK3	FC
PerCP anti-human CD4	SK3	FC
Spark Violet™ 538 anti-human CD4 Antibody	SK3	FC
Spark YG™ 581 anti-human CD4	SK3	FC
Alexa Fluor® 660 anti-human CD4	SK3	FC
Spark YG™ 593 anti-human CD4	SK3	FC
PE/Fire™ 810 anti-human CD4 Antibody	SK3	FC
APC/Fire™ 750 anti-human CD4	SK3	FC
Spark Blue™ 574 anti-human CD4	SK3	FC
GMP APC anti-human CD4	SK3	FC
TotalSeq™-B0045 anti-human CD4 Antibody	SK3	PG
Spark Violet™ 423 anti-human CD4 Antibody	SK3	FC
GMP Pacific Blue™ anti-human CD4	SK3	FC
Spark UV™ 387 anti-human CD4	SK3	FC
GMP PE anti-human CD4	SK3	FC
GMP FITC anti-human CD4	SK3	FC
Spark Red™ 718 anti-human CD4	SK3	FC
GMP PerCP anti-human CD4	SK3	FC
GMP PE/Cyanine7 anti-human CD4	SK3	FC
Spark Violet™ 500 anti-human CD4	SK3	FC
GMP PerCP/Cyanine5.5 anti-human CD4	SK3	FC
Brilliant Violet 650™ anti-human CD4	SK3	FC
PerCP/Fire™ 806 anti-human CD4	SK3	FC
Spark Blue™ 515 anti-human CD4	SK3	FC
GMP APC/Fire™ 750 anti-human CD4	SK3	FC
Spark Violet™ 423 anti-human CD4	SK3	FC
PerCP/Fire™ 780 anti-human CD4	SK3	FC
PE/Fire™ 744 anti-human CD4	SK3	FC
Spark PLUS UV395™ anti-human CD4	SK3	FC
Spark PLUS B550™ anti-human CD4	SK3	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 11/22/2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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