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PE/Fire™ 810 Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Streptavidin- PE/Fire™, SAv- PE/Fire™ 810, streptavidin PE/Fire™ 810
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Product Citations
publications
Streptavidin_PEFire810_Antibody_060823
Human peripheral blood lymphocytes were stained with biotinylated anti-human CD3, followed by SAV-PE/Fire™ 810 (filled histogram), or biotinylated mouse IgG1, κ isotype control, followed with SAV-PE/Fire™ 810 (open histogram).
  • Streptavidin_PEFire810_Antibody_060823
    Human peripheral blood lymphocytes were stained with biotinylated anti-human CD3, followed by SAV-PE/Fire™ 810 (filled histogram), or biotinylated mouse IgG1, κ isotype control, followed with SAV-PE/Fire™ 810 (open histogram).
  • Streptavidin_PEFire810_Antibody_2_060823
    Human peripheral blood lymphocytes were stained with anti-human CD8 FITC and biotinylated anti-human CD3 (left) or biotinylated mouse IgG1, κ isotype control (right), followed with SAV-PE/Fire™ 810.
See PE/Fire™ 810 spectral data
Cat # Size Price Quantity Check Availability Save
405367 25 µg $171
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Description

Streptavidin is a 52.8 kDa tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used to detect biotinylated proteins in a wide range of applications.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
Streptavidin is conjugated with PE/Fire™ 810 under optimal conditions.
Concentration
0.2 mg/mL (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin PE/Fire™ 810 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the PE/Fire™ 810 conjugation. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire™ 810 has a maximum excitation of 488/561 nm and a maximum emission of 810 nm.


Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect PE/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Related FAQs

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Go To Top Version: 1    Revision Date: 06/08/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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