PE/Dazzle 594 anti-human CD62P (P-Selectin) Antibody anti-CD62P

Pricing & Availability

Clone: AK4 (See other available formats)
Regulatory Status: RUO
Workshop: VI P-44
Other Names: GMP-140, PADGEM
Isotype: Mouse IgG1, κ
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Product Citations: publications

AK4_PEDZL594_CD62P_P-Selectin_Antibody_062817 — Thrombin-activated human peripheral blood platelets were stained with CD62P (clone AK4) PE/Dazzle™ 594 (filled histogram) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (open histogram).

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304929

25 tests

$147

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304930

100 tests

$358

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Description

CD62P is a 140 kD type I transmembrane glycoprotein also known as P-selectin, platelet activation-dependent granule membrane protein (PADGEM), and GMP-140. It is expressed on activated platelets, megakaryocytes, and endothelial cells. CD62P is primarily stored in secretory α-granules in platelets and Weibel-Palade bodies in endothelial cells, and is rapidly relocated to the plasma membrane upon activation. The ligands for CD62P are CD162 and CD24. A primary function of CD62P is cell adhesion during neutrophil rolling, and platelet-neutrophil and platelet-monocyte interactions.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections⁴ and in vitro blocking of adhesion of platelets¹. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 304947 & 304948).
RRID: AB_2687349 (BioLegend Cat. No. 304929)
AB_2687349 (BioLegend Cat. No. 304930)

Antigen Details

Structure: Selectin, type I glycoprotein, 140 kD
Distribution: Activated platelets, megakaryocytes, endothelial cells
Function: Adhesion, neutrophil rolling, platelet-neutrophil and platelet-monocyte interactions
Ligand/Receptor: CD162 (PSGL-1), CD24 and sialylated Lewis X
Cell Type: Endothelial cells, Megakaryocytes, Neutrophils, Platelets
Biology Area: Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. McEver R, et al. 1995. J. Biol. Chem. 270:11025.
2. Varki A. 1994. P. Natl. Acad. Sci. USA 91:7390.
Gene ID: 6403 View all products for this Gene ID
UniProt: View information about CD62P on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Agranulocyte Adhesion and Diapedesis

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Other Formats

View All CD62P Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD62P (P-Selectin)	AK4	FC
FITC anti-human CD62P (P-Selectin)	AK4	FC
PE anti-human CD62P (P-Selectin)	AK4	FC
PE/Cyanine5 anti-human CD62P (P-Selectin)	AK4	FC
Purified anti-human CD62P (P-Selectin)	AK4	FC,IHC-F
Biotin anti-human CD62P (P-Selectin)	AK4	FC
Alexa Fluor® 488 anti-human CD62P (P-Selectin)	AK4	FC
Alexa Fluor® 647 anti-human CD62P (P-Selectin)	AK4	FC
Brilliant Violet 605™ anti-human CD62P (P-Selectin)	AK4	FC
Brilliant Violet 421™ anti-human CD62P (P-Selectin)	AK4	FC
PE/Cyanine7 anti-human CD62P (P-Selectin)	AK4	FC
PerCP/Cyanine5.5 anti-human CD62P (P-Selectin)	AK4	FC
Alexa Fluor® 700 anti-human CD62P (P-Selectin)	AK4	FC
PE/Dazzle™ 594 anti-human CD62P (P-Selectin)	AK4	FC
APC/Fire™ 750 anti-human CD62P (P-Selectin)	AK4	FC
TotalSeq™-A0218 anti-human CD62P (P-Selectin)	AK4	PG
Brilliant Violet 650™ anti-human CD62P (P-Selectin)	AK4	FC
Brilliant Violet 510™ anti-human CD62P (P-Selectin)	AK4	FC
Brilliant Violet 711™ anti-human CD62P (P-Selectin)	AK4	FC
APC/Cyanine7 anti-human CD62P (P-Selectin)	AK4	FC
Brilliant Violet 785™ anti-human CD62P (P-Selectin)	AK4	FC
TotalSeq™-C0218 anti-human CD62P (P-Selectin)	AK4	PG
Ultra-LEAF™ Purified anti-human CD62P (P-Selectin)	AK4	FC
TotalSeq™-B0218 anti-human CD62P (P-Selectin) Antibody	AK4	PG
TotalSeq™-D0218 anti-human CD62P (P-Selectin)	AK4	PG
PE anti-human CD62P	AK4	FC
FITC anti-human CD62P	AK4	FC
GMP FITC anti-human CD62P (P-Selectin)	AK4	FC
GMP PE anti-human CD62P (P-Selectin)	AK4	FC

Certificate of Analysis

Go To Top Version: 0 Revision Date: 06/28/2017

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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