PE/Fire™ 700 anti-human CD3 Antibody, CD3, SK7

Pricing & Availability

Clone: SK7 (See other available formats)
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: T3, CD3ε
Isotype: Mouse IgG1, κ
Ave. Rating: Submit a Review
Product Citations: publications

SK7_PEFire700_CD3_Antibody_071420 — Human peripheral blood lymphocytes were stained with anti-human CD19 FITC and anti-human CD3 PE/Fire™ 700 (clone SK7) (left), or mouse IgG1, κ PE/Fire™ 700 (right).

Compare all formats See PE/Fire™ 700 spectral data

Cat #

Size

Price

Quantity Check Availability

Save

344863

25 tests

$188

Check Availability

Need larger quantities of this item?
Request Bulk Quote

344864

100 tests

$440

Check Availability

Need larger quantities of this item?
Request Bulk Quote

Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 700 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire™ 700 has a maximum excitation of 565 nm and a maximum emission of 695 nm.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections^4,5,8, immunofluorescent staining⁶, and Western blotting³.
RRID: AB_2876653 (BioLegend Cat. No. 344863)
AB_2876653 (BioLegend Cat. No. 344864)

Antigen Details

Structure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ, (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution: Mature T and NK T cells, during thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand/Receptor: Peptide antigen bound to MHC
Cell Type: NKT cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501.
Gene ID: 916 View all products for this Gene ID
UniProt: View information about CD3 on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD3 Reagents Request Custom Conjugation

Description	Clone	Applications
APC/Fire™ 750 anti-human CD3	SK7	FC
Biotin anti-human CD3	SK7	FC,ICC
Purified anti-human CD3	SK7	FC,ICC,IHC-F,WB
FITC anti-human CD3	SK7	FC
PE anti-human CD3	SK7	FC
Alexa Fluor® 488 anti-human CD3	SK7	FC
APC anti-human CD3	SK7	FC
PerCP/Cyanine5.5 anti-human CD3	SK7	FC
PerCP anti-human CD3	SK7	FC
PE/Cyanine7 anti-human CD3	SK7	FC
APC/Cyanine7 anti-human CD3	SK7	FC
Alexa Fluor® 700 anti-human CD3	SK7	FC
Pacific Blue™ anti-human CD3	SK7	FC
Alexa Fluor® 647 anti-human CD3	SK7	FC
Brilliant Violet 510™ anti-human CD3	SK7	FC
Brilliant Violet 421™ anti-human CD3	SK7	FC
Brilliant Violet 605™ anti-human CD3	SK7	FC
Brilliant Violet 711™ anti-human CD3	SK7	FC
FITC anti-human CD3	SK7	FC
PE anti-human CD3	SK7	FC
Brilliant Violet 785™ anti-human CD3	SK7	FC
PE/Dazzle™ 594 anti-human CD3	SK7	FC
Brilliant Violet 750™ anti-human CD3	SK7	FC
TotalSeq™-A0049 anti-human CD3	SK7	PG
PerCP/Cyanine5.5 anti-human CD3	SK7	FC
TotalSeq™-C0049 anti-human CD3	SK7	PG
Spark Blue™ 550 anti-human CD3	SK7	FC
PE/Cyanine7 anti-human CD3	SK7	FC
TotalSeq™-B0049 anti-human CD3	SK7	PG
Alexa Fluor® 660 anti-human CD3	SK7	FC
APC/Fire™ 810 anti-human CD3	SK7	FC
Spark NIR™ 685 anti-human CD3	SK7	FC
PE/Fire™ 640 anti-human CD3	SK7	FC
PE/Fire™ 700 anti-human CD3	SK7	FC
APC anti-human CD3	SK7	FC
PerCP anti-human CD3	SK7	FC
APC/Fire™ 750 anti-human CD3	SK7	FC
GMP FITC anti-human CD3	SK7	FC
PE/Cyanine5 anti-human CD3 Antibody	SK7	FC
GMP PE anti-human CD3	SK7	FC
GMP APC anti-human CD3	SK7	FC
GMP PerCP/Cyanine5.5 anti-human CD3	SK7	FC
Spark YG™ 593 anti-human CD3	SK7	FC
GMP PerCP anti-human CD3	SK7	FC
Spark Violet™ 500 anti-human CD3	SK7	FC
Brilliant Violet 650™ anti-human CD3	SK7	FC
Spark Violet™ 500 anti-human CD3	SK7	FC
Spark PLUS UV395™ anti-human CD3	SK7	FC

Certificate of Analysis

Customers Also Purchased

PE/Cyanine7 anti-human CD314 (NKG2D)

Clone: 1D11

APC/Fire™ 750 anti-human CD279 (PD-1)

Clone: EH12.2H7

Brilliant Violet 421™ anti-human CD223 (LAG-3)

Clone: 11C3C65

Brilliant Violet 605™ anti-human CD15 (SSEA-1)

Clone: W6D3

Go To Top Version: 2 Revision Date: 08/27/2020

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.