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PerCP/Cyanine5.5 anti-human β2-microglobulin Antibody

Pricing & Availability
Clone
A17082E (See other available formats)
Regulatory Status
RUO
Other Names
β2M, β2-M, beta2-microglobulin b2-M, b2M
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A17082E_PerCPCyanine55_B2-microglobulin_Antibody_051319
Human peripheral blood lymphocytes were stained with ß2-microglobulin (clone A17082E) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • A17082E_PerCPCyanine55_B2-microglobulin_Antibody_051319
    Human peripheral blood lymphocytes were stained with ß2-microglobulin (clone A17082E) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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395809 25 tests $176
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395810 100 tests $382
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Description

β2-microglobulin (β2M) is a 12 kD nonpolymorphic Ig like protein. It is a non-membrane-anchored glycoprotein and is noncovalently associated with 39-44 kD polymorphic heavy chains of MHC class I molecules to form HLA class I antigen complex. In association with HLA class I, β2M is expressed on all leukocytes, platelets, endothelial cells, and epithelial cells. β2M plays an essential role both in governing MHC class I molecules stability and in promoting antigen binding and presenting the antigen to CD3/TCR complex of CD8+ T cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Beta2-microglobulin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Based on in-house testing, staining for clone A17082E is not blocked by clone W6/32 (anti-HLA-A,B,C) and is only partially blocked by clone 2M2 also raised against human ß2-microglobulin.

RRID
AB_2810595 (BioLegend Cat. No. 395809)
AB_2810595 (BioLegend Cat. No. 395810)

Antigen Details

Structure
12KD, Ig like structure
Distribution

Leukocytes, platelets, endothelial cells, epithelial cells and other nucleated cells.

Function
Form MHC class I
Interaction
MHC class I heavy chain and other MHC I like molecules.
Cell Type
Endothelial cells, Epithelial cells, Leukocytes, Platelets
Antigen References
  1. Engelhard VH. 1994. Curr Opin Immunol. 6:13.
  2. Williams DB, et al. 1989. J Immunol. 142:2796.
  3. Danliczyk UG and TL. Delovitch. 1994. J Immunol. 153:3533.
  4. Williams A, et al. 2002. Tissue Antigens. 59:3.
Gene ID
567 View all products for this Gene ID
UniProt
View information about beta2-microglobulin on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 05/13/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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