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PerCP/Cyanine5.5 anti-human CD23 Antibody

Pricing & Availability
Clone
EBVCS-5 (See other available formats)
Regulatory Status
RUO
Other Names
Leu-20, FcεRII, IgE Fc Receptor, BLAST-2, B6, Low affinity IgE receptor
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
EBVCS-5_PerCPCyanine55_CD23_Antibody_030619_updated.png
Human peripheral blood lymphocytes were stained with CD19 APC and CD23 (clone EBVCS-5) PerCP/Cyanine5.5 (left) or Mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right)
  • EBVCS-5_PerCPCyanine55_CD23_Antibody_030619_updated.png
    Human peripheral blood lymphocytes were stained with CD19 APC and CD23 (clone EBVCS-5) PerCP/Cyanine5.5 (left) or Mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right)
Compare all formats See PerCP/Cyanine5.5 spectral data
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338517 25 tests $176
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338518 100 tests $382
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Description

CD23 is a 45 kD protein, also known as Leu-20, FcεRII, IgE Fc receptor, BLAST-2, B6, and low affinity IgE receptor. It is a member of the Ig family, expressed on most mature B cells, B cells in follicular mantle (but not in proliferating germinal center cells, follicular dendritic cells, monocytes, eosinophils, Langerhans cells, and a subset of T cells (10-15% of tonsillar T cells). CD23 responds to high levels of IgE by downregulating IgE secretion. In human monocytes, CD23 triggering results in release of pro-inflammatory cytokines including TNF-α, IL-1, IL-6, and GM-CSF. CD23 can be proteolytically cleaved to generate soluble CD23 fragments of various molecular weights. In chronic lymphocytic leukemia, levels of soluble CD23 in the serum can be used as a prognostic marker to identify patients at high risk for disease progression. Alternate splicing of exon 2 can also generate two cell-surface isoforms of CD23 differing by 6 amino acids in their cytoplasmic region.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and 564 nm and a maximun emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Sugden B and Metzenberg S. 1983. J. Virol. 46:800-807.
Product Citations
  1. Nygren P, et al. 2019. Cell Microbiol. e12967:21. PubMed
  2. Deng C, et al. 2016. Diabetes Care. 39: 434 - 440. PubMed
  3. Deng C, et al. 2017. J Diabetes Res. 10.1155/2017/5052812. PubMed
  4. Krishnan S, et al. 2021. Clin Exp Immunol. 203:458. PubMed
RRID
AB_1501120 (BioLegend Cat. No. 338517)
AB_1501120 (BioLegend Cat. No. 338518)

Antigen Details

Structure
Ig family member, type II transmembrane glycoprotein, 45 kD
Distribution

B cells, follicular dendritic cells, monocytes, eosinophils, a subset of T cells, Langerhans cells

Function
Regulation of IgE synthesis in human monocytes; CD23 triggering results in release of pro-inflammatory cytokines including TNF-a, IL-1, IL-6, and GM-CSF
Ligand/Receptor
IgE, CD19/CD21/CD81
Cell Type
B cells, Dendritic cells, Eosinophils, Langerhans cells, Monocytes, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Ludin C, et al. 1987. EMBO J. 6:109.
2. Delespesse G, et al. 1992. Immunol. Rev. 125:77.
3. Flores-Romo L, et al. 1993. Science 261:1038.
4. Armant M, et al. 1994. J. Exp. Med. 180:1005.

Gene ID
2208 View all products for this Gene ID
UniProt
View information about CD23 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 03/05/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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