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PerCP/Cyanine5.5 anti-human CD369 (Dectin-1/CLEC7A) Antibody

Pricing & Availability
Clone
15E2 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
CLECSF12, beta-glucan receptor (BGR, betaGR), CD369
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
15E2_PerCPCyanine5dot5_CD369_Antibody_031119.png
Human peripheral blood monocytes were stained with anti-human CD369 (Dectin-1/CLEC7A) (clone 15E2) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • 15E2_PerCPCyanine5dot5_CD369_Antibody_031119.png
    Human peripheral blood monocytes were stained with anti-human CD369 (Dectin-1/CLEC7A) (clone 15E2) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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355407 25 tests $165
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355408 100 tests $422
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Description

CD369 (Dectin-1), also known as CLEC7A and beta-glucan receptor (BGR), is a 33 kD type II transmembrane C-type lectin. It consists of a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain, a transmembrane segment, and a short N-terminal ITAM containing a cytoplasmic tail. CLEC7A is predominantly expressed on dendritic cells. Subsequent work has shown expression on B cells, monocytes, eosinophils, mast cells, macrophages, endothelial cells, and low expression on some subsets of T cells. CLEC7A lacks residues involved in calcium ligation that mediates carbohydrate-binding by classical C-type lectins. CLEC7A functions as a receptor to mediate innate immunity to fungal pathogens by selectively binding beta-glucan polymers, a major component of yeast and mycobacterial cell walls. 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Ectodomain with C-term hFc fusion, RIBLS.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The epitope of clone 15E2 is LWEDGSTFSSN. Clone 15E2 can recognize the epitope in the presence of Curdlan and Laminarin (CLEC7A ligands). Additional reported applications (for the relevant formats) include: immunohistochemical staining.1

Application References

(PubMed link indicates BioLegend citation)
  1. Ni L, et al. 2010. J. Immunol. 185:3504. (IHC)
RRID
AB_2800958 (BioLegend Cat. No. 355407)
AB_2800958 (BioLegend Cat. No. 355408)

Antigen Details

Structure
Type II transmemberane C-type lectin, one carbohydrate recognition domain, ITAM containing cytoplasmic tail
Distribution

B cells, low level of some subsets of T cells, monocytes, dendritic cells, macrophages and endothelial cells

Function
Mediates innate immunity to fungal pathogens
Interaction
TLR2, CD37
Ligand/Receptor
Beta-glucan
Cell Type
B cells, Dendritic cells, Endothelial cells, Macrophages, Monocytes, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Goodridge HS, et al. 2011. Nature 472:471.
2. Saijo S, et al. 2007. Nat. Immunol. 8:39.
3. Brown GD. 2006. Nat. Rev. Immunol. 6:33.
4. Willment JA, et al. 2001. J. Biol. Chem. 276:43818.
5. Yokota K, et al. 2001. Gene 272:51.

Gene ID
64581 View all products for this Gene ID
UniProt
View information about CD369 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 03/11/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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