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PerCP/Cyanine5.5 anti-human CD68 Antibody

Pricing & Availability
Clone
Y1/82A (See other available formats)
Regulatory Status
RUO
Workshop
VI MR23
Other Names
Macrosialin
Isotype
Mouse IgG2b, κ
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Product Citations
publications
Y1slash82A_PerCPCyanine55_CD68_Antibody_110718
Human peripheral blood mononuclear cells were fixed, permeabilized and intracellularly stained with anti-human CD68 (clone Y1/82A) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2b, κ PerCP/Cyanine5.5 isotype control (open histogram). Data shown was gated on the monocyte population.
  • Y1slash82A_PerCPCyanine55_CD68_Antibody_110718
    Human peripheral blood mononuclear cells were fixed, permeabilized and intracellularly stained with anti-human CD68 (clone Y1/82A) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2b, κ PerCP/Cyanine5.5 isotype control (open histogram). Data shown was gated on the monocyte population.
Compare all formats See PerCP/Cyanine5.5 spectral data
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333813 25 tests $176
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333814 100 tests $382
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Description

CD68 is a 110 kD glycoprotein, also known as macrosialin, belonging to the sialomucin family. It is closely related to the family of acidic, highly glycosylated lysosomal-associated membrane proteins (LAMPs). CD68 is predominately expressed in cytoplasmic granules of monocytes/macrophages, dendritic cells, and granulocytes. It is one of the useful myeloid cell markers. Further studies have shown that CD68 is also expressed by a subset of hematopoietic progenitors, γ/δ T cells, NK cells, LAK cells, subset of B cells, fibroblasts, and endothelial cells. The biological function of CD68 is still unknown.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported application: immunohistochemical staining of frozen tissue sections. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. Doussis IA, et al. 1993. J. Clin. Pathol. 46:334.
  2. Davey FR, et al. 1988. J. Clin. Pathol. 41:753.
  3. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
Product Citations
  1. Hohmann MS, et al. 2021. JCI Insight. 6:. PubMed
  2. Wu X, et al. 2022. Front Immunol. 13:811551. PubMed
  3. Bohrer AC, et al. 2022. Cell Rep. 40:111144. PubMed
  4. Bayik D, et al. 2022. Cancer Res. 82:4274. PubMed
  5. Liu X, et al. 2023. J Transl Med. 21:314. PubMed
  6. Zhang Y, et al. 2020. Oncol Lett. 1.053472222. PubMed
  7. Khoonrit P, et al. 2020. PLoS One. 15:e0238509. PubMed
  8. Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed
  9. Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed
  10. Wu X, et al. 2018. J Immunol Methods. 455:71. PubMed
  11. Patel C, et al. 2022. Curr Protoc. 2:e400. PubMed
RRID
AB_10681723 (BioLegend Cat. No. 333813)
AB_10681723 (BioLegend Cat. No. 333814)

Antigen Details

Structure
Sialomucin family, 110 kD
Distribution

Monocytes/macrophages, dendritic cells, granulocytes, subset of hematopoietic progenitors, γ/δ T cells, NK cells, LAK cells, subset of B cells, fibroblasts, endothelial cells

Cell Type
B cells, Dendritic cells, Endothelial cells, Fibroblasts, Granulocytes, Hematopoietic stem and progenitors, Macrophages, Monocytes, T cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Holness CL and Simmons DL. 1993. Blood 81:1607.
2. Gottfried E, et al. 2008. Scand. J. Immunol. 67:453.
3. Hameed A, et al. 1994. Hum. Pathol. 25:872.

Gene ID
968 View all products for this Gene ID
UniProt
View information about CD68 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 11/07/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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