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PerCP/Cyanine5.5 anti-human/mouse Bcl-6 Antibody

Pricing & Availability
Clone
7D1 (See other available formats)
Regulatory Status
RUO
Other Names
B-cell lymphoma 6, BCL5, LAZ3, BCL6A, ZNF51, ZBTB27
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
7D1_PerCPCy5.5_Bcl-6_Antibody_FC_081915
Burkitt's lymphoma cell line, Ramos, was stained with the True-Nuclear™ Transcription Factor Buffer Set, and stained with Bcl-6 (clone 7D1) PerCP/Cyanine5.5 (filled histogram) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • 7D1_PerCPCy5.5_Bcl-6_Antibody_FC_081915
    Burkitt's lymphoma cell line, Ramos, was stained with the True-Nuclear™ Transcription Factor Buffer Set, and stained with Bcl-6 (clone 7D1) PerCP/Cyanine5.5 (filled histogram) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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358507 25 tests $171
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358508 100 tests $405
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Description

B-cell lymphoma 6 (Bcl-6), is an 80 kD homodimer, member of the BTB-POZ zinc finger family. It contains 1 BTB (POZ) domain and 6 C2H2-type zinc fingers. Bcl-6 is a transcriptional repressor, master regulator of germinal center reaction. On B cells, Bcl-6 induces proliferation, antibody class switch and affinity maturation, while inhibits its differentiation to plasma cells. On T cells, Bcl-6 induces its differentiation to TFH. This molecule is also expressed in some B cell lymphomas and breast cancer cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Sequence 226-398 of murine BCL6 fused with GST
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1,2,3.

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Vikstrom, et al. 2010. Science 330:1095. (WB)
  2. Kallies A, et al. 2011. Blood 117:1869. (WB)
  3. Lüthje K, et al. 2012. Nat. Immunol. 13:491. (WB)
  4. Tonti E, et al. 2012. J. Immunol. 188:3217. (ICFC)
Product Citations
  1. Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed
  2. Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
RRID
AB_2566191 (BioLegend Cat. No. 358507)
AB_2566191 (BioLegend Cat. No. 358508)

Antigen Details

Structure
Member of the BTB-POZ zinc finger family, 80 kD, homodimer, 1 BTB (POZ) domain, 6 C2H2-type zinc fingers
Distribution

Germinal center B cells, follicular helper T cells (TFH), B cell lymphomas, breast cancer cells

Function
Transcription factor, master regulator of germinal center reaction
Interaction
BLIMP-1
Cell Type
B cells, Tfh
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Basso K and Dalla-Favera R. 2012. Immunol. Rev. 247:172.
2. Vinuesa CG, and Cyster JG, 2011. Immunity 35:671.
3. Kitano M, et al. 2011. Immunity 34:961.
4. Baumjohann D, et al. 2011. J. Immunol. 187:2089.
5. Johnston RJ, et al. 2009. Science. 325:1006.
6. Tran TH, et al. 2010. Cancer Res. 70:1711.

Gene ID
604 View all products for this Gene ID
UniProt
View information about Bcl-6 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 05/24/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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