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PerCP/Cyanine5.5 anti-human TCR Cβ1 Antibody

Pricing & Availability
Clone
JOVI.1 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor beta constant 1, TRBC1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
JOVI1_PerCPCyanine55_TCR_Cbeta1_Antibody_091322
Human peripheral blood lymphocytes were stained with anti-human CD3 FITC and anti-human TCR Cβ1 (clone JOVI.1) PerCP/Cyanine5.5 (left) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
  • JOVI1_PerCPCyanine55_TCR_Cbeta1_Antibody_091322
    Human peripheral blood lymphocytes were stained with anti-human CD3 FITC and anti-human TCR Cβ1 (clone JOVI.1) PerCP/Cyanine5.5 (left) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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383507 25 tests $193
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383508 100 tests $396
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Description

TRBC1 is a 20 kD protein constant region of the T cell receptor also known as T cell receptor beta constant 1. It is expressed on a fraction of TCRαβ T cells on the beta chain of the T Cell receptor. The constant region expression of clones is notable as a marker for T Cell disease development by clonality detection.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Transgenic mouse lymphocytes expressing human Vβ3-Cβ1 TCR
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone JOVI.1 can interfere binding of clone IP26 on target cells.

Application References

(PubMed link indicates BioLegend citation)
  1. Viney, J L et al. 1992. Hybridoma. 11:701-13.
  2. Horna, Pedro et al. 2021. Int J Mol Sci. 22:1817.
RRID
AB_2927883 (BioLegend Cat. No. 383507)
AB_2927883 (BioLegend Cat. No. 383508)

Antigen Details

Structure
Predicted 20 kD Ig-like protein
Distribution

T lymphocytes

Cell Type
Leukemia, Lymphocytes, T cells, Thymocytes
Biology Area
Cancer Biomarkers, Cell Biology, Immunology
Molecular Family
TCRs
Antigen References
  1. Minden, M D and T W Mak. 1986. Blood. 68:327-36.
  2. Davis, M M et al. 1997. Ciba Found Symp. 204:94-100.
  3. Maciocia, Paul M et al. 2017. Nat Med. 23:1416-23.
Gene ID
28639 View all products for this Gene ID
UniProt
View information about TCR Cbeta1 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 09/13/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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