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PerCP/Cyanine5.5 anti-MEK1 Phospho (Ser298) Antibody

Pricing & Availability
Clone
A16117B (See other available formats)
Regulatory Status
RUO
Other Names
MEK1, MAP2K1, CFC3, MKK1, MAPKK1, PRKMK1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A16117B_PerCPCyanine5_Phospho_Ser298_Antibody_111120
Human peripheral blood lymphocytes were stimulated with (filled histogram) or without (open histogram) Cell Activation Cocktail (without Brefeldin A) for 15 minutes, then fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, and intracellularly stained with anti-MEK1 Phospho (Ser298) antibody (clone A16117B) PerCP/Cyanine5.5.
  • A16117B_PerCPCyanine5_Phospho_Ser298_Antibody_111120
    Human peripheral blood lymphocytes were stimulated with (filled histogram) or without (open histogram) Cell Activation Cocktail (without Brefeldin A) for 15 minutes, then fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, and intracellularly stained with anti-MEK1 Phospho (Ser298) antibody (clone A16117B) PerCP/Cyanine5.5.
Compare all formats See PerCP/Cyanine5.5 spectral data
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610605 25 tests $176
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610606 100 tests $393
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Description

MEK1 and MEK2 (Map/Erk Kinase) are serine/threonine kinases in the MAPK/ERK signaling axis that play a critical role in cellular proliferation, differentiation, and migration. Phosphorylation at MEK1 (Ser298) by PAK1 enhances the ability of upstream Raf kinases to phosphorylate regulatory serines (Ser218 and Ser222) in the activation loop, thereby stimulating kinase activity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human MEK1 peptide phosphorylated at Ser298. Complete Freund’s adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
RRID
AB_2888736 (BioLegend Cat. No. 610605)
AB_2888736 (BioLegend Cat. No. 610606)

Antigen Details

Structure
MEK1 is a 393 amino acid protein with a predicted molecular weight of 43 kD
Distribution

Ubiquitously expressed/cytosolic

Biology Area
Cell Biology, Cell Proliferation and Viability, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References
  1. Song C, et al. 2017. Sci Rep. 7:46833.
  2. Jeong DE, et al. 2017. EMBO J. 36:1046.
  3. Kyriakakis E, et al. 2017. Cell Signal. 35:163.
  4. Narbonne P, et al. PLoS Genet. 13:e1006738.
  5. Wu H, et al. 2013. PLoS One. 8:e83737.
  6. Wang Z, et al. 2010. Oncogene. 29:3362.
  7. Moniz S, et al. 2007. Oncogene. 26:6071.
  8. Park ER, et al. 2007. Cell Signal. 7:1488.
Gene ID
5604 View all products for this Gene ID
UniProt
View information about MEK1 Phospho Ser298 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 11/11/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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