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PerCP/Cyanine5.5 anti-mouse CD115 (CSF-1R) Antibody

Pricing & Availability
Clone
AFS98 (See other available formats)
Regulatory Status
RUO
Other Names
CSF-1R, M-CSFR, c-fms
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
AFS98_PerCPCyanine55_CD115_Antibody_082218
Thioglycolate-elicited BALB/c peritoneal macrophages were stained with CD11b FITC and CD115 (CSF-1R, clone AFS98) PerCP/Cyanine5.5 (left), or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
  • AFS98_PerCPCyanine55_CD115_Antibody_082218
    Thioglycolate-elicited BALB/c peritoneal macrophages were stained with CD11b FITC and CD115 (CSF-1R, clone AFS98) PerCP/Cyanine5.5 (left), or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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135525 25 µg $124
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135526 100 µg $335
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Description

CSF-1R, also known as CD115 and M-CSFR, is a single-pass type I membrane protein and member of the platelet-derived growth factor receptor family. This c-fms (Fms proto-oncogene) gene product's natural ligands include M-CSF and IL-34. Structural studies of CD115 have described an Ig-like extracellular domain, a transmembrane domain, an intracellular juxtamembrane domain, a split tyrosine kinase domain, and a C-terminal tail receptor. Receptor activation induces homodimerization in addition to phosphorylation and ubiquitination of intracellular residues. CD115 directly influences tissue macrophage and osteoclast differentiation and proliferation. It is expressed on monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, and osteoclasts.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of ligand binding1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.

It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation. Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD1154.

Application References

(PubMed link indicates BioLegend citation)
  1. Sudo T, et al. 1995. Oncogene 11:2469.
  2. Murayama T, et al. 1999. Circulation 99:1740.
  3. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. PubMed
  4. Breslin WL, et al. 2013. J Immunol Methods. 390(1-2):1 PubMed
  5. Dong L, et al. 2016. Nature. 539:304–308. PubMed
Product Citations
  1. Li X, et al. 2022. Cell. 185:1709. PubMed
  2. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
  3. Ramakrishna C, et al. 2019. Nat Commun. 10:2153. PubMed
  4. Wu Z, et al. 2023. EMBO Rep. 24:e56524. PubMed
  5. Vanneste D, et al. 2023. Nat Immunol. 24:827. PubMed
  6. Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
  7. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  8. Ali S, et al. 2021. PLoS One. 16:e0246646. PubMed
  9. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  10. Hilt ZT, et al. 2019. JCI Insight. 4:5. PubMed
RRID
AB_2566461 (BioLegend Cat. No. 135525)
AB_2566461 (BioLegend Cat. No. 135526)

Antigen Details

Structure
Ig superfamily, 145 kD
Distribution

Monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, osteoclasts

Function
Growth factor receptor, tyrosine kinase
Ligand/Receptor
Macrophage colony stimulating factor (M-CSF), IL-34
Cell Type
Dendritic cells, Macrophages, Monocytes, Osteoclasts
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Sudo T, et al. 1995 Oncogene 11:2469.
  2. Murayama T, et al. 1999 Circulation 99:1740.
  3. Goswami S, et al. 2005 Cancer Res. 65:5278.
  4. Yu W, et al. 2008 J. Leuko. Biol. 84:852.
Gene ID
12978 View all products for this Gene ID
UniProt
View information about CD115 on UniProt.org

Related FAQs

Why do I have a weak CD115 staining?

It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation.  Pre-treatment with EDTA and low temperatures (2 - 8°C) helps in maintaining surface expression of CD115. In addition, brief fixation of the cells with Fixation Buffer (Cat. No. 420801) for 10 minutes will block CD115 internalization.

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 08/22/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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