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PerCP/Cyanine5.5 anti-mouse CD205 (DEC-205) Antibody

Pricing & Availability
Clone
NLDC-145 (See other available formats)
Regulatory Status
RUO
Other Names
DEC-205
Isotype
Rat IgG2a, κ
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Product Citations
publications
NLDC-145_PerCPCyanine55_CD205_Antibody_1_030624
C57BL/6 mouse bone marrow cells stained with CD205 (clone NLDC-145) PerCP/Cyanine5.5 and Ly-6G/Ly-6C (clone RB6-8C5) APC.
  • NLDC-145_PerCPCyanine55_CD205_Antibody_1_030624
    C57BL/6 mouse bone marrow cells stained with CD205 (clone NLDC-145) PerCP/Cyanine5.5 and Ly-6G/Ly-6C (clone RB6-8C5) APC.
Compare all formats See PerCP/Cyanine5.5 spectral data
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138207 25 µg $129
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138208 100 µg $382
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Description

CD205, also known as DEC-205, is a 205 kD integral membrane protein homologous to the macrophage mannose receptor. It is a type I cell surface protein that belong to the C-type lectin family.  CD205 is expressed at high levels by dendritic cells and thymic epithelial cells.  It is also expressed by a number of other cell types, such as B lymphocytes, macrophages, Langerhans cells, bone marrow stromal cells, granulocytes, epithelial cells of pulmonary airways, and the capillaries of the brain.  CD205 is a novel endocytic receptor used by dendritic cells and thymic epithelial cells to direct captured antigens from the extracellular space to specialized antigen processing.  It mediates antigen uptake and presentation and cross-presentation to T cells.  It has been reported that CD205 acts as a recognition receptor for dying cells, potentially provides an important pathway for the uptake of self-antigen in the intrathymic environment, and is involved in peripheral tolerance.  Antibody-mediated antigen-targeting via the DEC-205 receptor increases the efficiency of vaccination for T cell immunity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse lymph node tissue
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

 
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1, Western Blot1-3, immunoprecipitation of bone marrow dendritic cell extracts2, and spatial biology (IBEX)6,7.

Application References

(PubMed link indicates BioLegend citation)
  1. Witmer-Pack MD, et al. 1995. Cell. Immun. 163:157. (IHC, WB)
  2. Swiggard WJ, et al. 1995. Cell. Immun. 165:302. (WB, IP)
  3. Bonifaz LC, et al. 2004. J. Exp. Med. 199:815. (WB)
  4. Yamazaki S, et al. 2002. J. Immunol. 181:6923. (FC)
  5. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC)
  6. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  7. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Singh VB, et al. 2015. J Neurovirol. 186:21. PubMed
RRID
AB_10641140 (BioLegend Cat. No. 138207)
AB_10641140 (BioLegend Cat. No. 138208)

Antigen Details

Structure
A 205 kD type I tramsmembrane protein belonging to the C-type lectin family and homologous to the macrophage mannose receptor.
Distribution

Expressed at high levels by dendritic cells and thymic epithelial cells. Also expressed by B lymphocytes, macrophages, Langerhans cells, bone marrow cells and epithelial cells.

Function
Mediates endocytosis, antigen uptake, presentation, and cross-presentation to T cells.
Cell Type
B cells, Dendritic cells, Epithelial cells, Langerhans cells, Macrophages
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Jiang WP, et al. 1995. Nature 375:151.
2. Small M and Kraal G. 2003. Int. Immunol. 15:197.
3. Shrimpton RE, et al. 2009. Mol. Immunol. 46:1229.

Gene ID
17076 View all products for this Gene ID
UniProt
View information about CD205 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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