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PerCP/Cyanine5.5 anti-mouse CD4 Antibody

Pricing & Availability
Clone
RM4-4 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
RM4-4_PerCpCyanine55_CD4_Antibody_110518
C57BL/6 mouse splenocytes were stained with CD3e FITC and CD4 (clone RM4-4) PerCP/Cyanine5.5 (left), or rat IgG2b, κ PerCP/Cyanine5.5 isotype control (right).
  • RM4-4_PerCpCyanine55_CD4_Antibody_110518
    C57BL/6 mouse splenocytes were stained with CD3e FITC and CD4 (clone RM4-4) PerCP/Cyanine5.5 (left), or rat IgG2b, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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116011 25 µg $101
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116012 100 µg $253
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Description

CD4 is a 55 kD protein, also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

RM4-4 antibody does not block the binding of GK1.5 and RM4-5 antibodies to CD4 T cells. For immunohistochemistry applications, the RM4-5 (Cat. No. 100506) and GK1.5 (Cat. No. 100402) antibodies are recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Bendelac A. 1995. Curr. Opin. Immunol. 7:367.
  2. Norian LA and Allen PM. 2004. J. Immunol. 173:835.
Product Citations
  1. Zhou R, et al. 2022. EBioMedicine. 75:103762. PubMed
  2. Tian J, et al. 2021. Autophagy. 17:2586. PubMed
  3. Wang R, et al. 2023. NPJ Vaccines. 8:6. PubMed
  4. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  5. Gilleron J et al. 2018. Cell reports. 25(12):3329-3341 . PubMed
  6. Stathopoulou C, et al. 2020. Immunity. 49(2):247-263.e7.. PubMed
  7. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  8. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  9. Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
  10. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  11. Ye P, et al. 2022. Front Cardiovasc Med. 8:810477. PubMed
  12. Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed
  13. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  14. Kyburz A, et al. 2017. Clin Exp Allergy. 47:1331. PubMed
  15. Gupta S, et al. 2022. Life Sci. 288:120182. PubMed
  16. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
RRID
AB_2563022 (BioLegend Cat. No. 116011)
AB_2563022 (BioLegend Cat. No. 116012)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579. 
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 11/05/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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