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PerCP/Cyanine5.5 anti-mouse/human KLRG1 (MAFA) Antibody

Pricing & Availability
Clone
2F1/KLRG1 (See other available formats)
Regulatory Status
RUO
Other Names
MAFA, 2F1-Ag
Isotype
Syrian Hamster IgG
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Product Citations
publications
2F1slashKLRG1_PerCPCyanine55_KLRG1_Antibody_101818
C57BL/6 mouse splenocytes were stained with NK-1.1 APC and anti-mouse/human KLRG1 (clone 2F1/KLRG1) PerCP/Cyanine5.5 (left), or Syrian hamster IgG PerCP/Cyanine5.5 isotype control (right).
  • 2F1slashKLRG1_PerCPCyanine55_KLRG1_Antibody_101818
    C57BL/6 mouse splenocytes were stained with NK-1.1 APC and anti-mouse/human KLRG1 (clone 2F1/KLRG1) PerCP/Cyanine5.5 (left), or Syrian hamster IgG PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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138417 25 µg $124
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138418 100 µg $335
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Description

Killer cell lectin-like receptor G1 (KLRG1) is the mouse homolog of the rat mast cell function-associated antigen (MAFA or 2F1-Ag).  KLRG1 is a type II membrane glycoprotein that was first identified on the surface of rat mast cell line RBL-2H3.  It is composed of a homodimer of glycosylated 30-38 kD subunits.  Mouse and human homologs of KLRG1 are expressed by subsets of NK cells and lymphokine-activated killer (LAK) cells but not mast cells.  KLRG1 is also expressed on subsets of CD8+ and CD4+ cells, including CD4+ and CD8+ effector/memory cells, potent regulatory CD4+ T cells.  KLRG1 may be involved in regulating NK cell homeostasis.  KLRG1 was found to recognize cadherins and thus inhibit immune responses by regulating the effector function and the developmental processes of NK and T cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Immunogen
IL-2 activated NK cells from C57BL/6 mice
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

For successful staining of human cells, brighter fluorophore-antibody conjugates, such as PE, BV421™, or APC, are recommended.

Additional reported applications (for relevant formats) include: Western Blotting1.

Application References

(PubMed link indicates BioLegend citation)
  1. Robbins SH, et al. 2002. J. Immunol. 168:2585. (WB)
  2. Robbins SH, et al. 2003. J. Immunol. 170:5876. (FC)
  3. McMahon CW, et al. 2002. J. Immunol. 169:1444. (FC)
Product Citations
  1. Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed
  2. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  3. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  4. Wang D, et al. 2018. Immunity. 48:659. PubMed
  5. Bromley SK, et al. 2020. Cell Reports. 32(9):108085. PubMed
  6. Zhang Y, et al. 2021. J Immunol Res. 2021:5599439. PubMed
  7. Van Gool F, et al. 2019. Immunity. 50:362. PubMed
  8. Moguche AO et al. 2017. Cell host & microbe. 21(6):695-706 . PubMed
  9. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  10. Vacca F, et al. 2020. eLife. 9:e54017.. PubMed
  11. Sema Kurtulus et al. 2018. Immunity. 50(1):181-194 . PubMed
  12. Glodde N et al. 2017. Immunity. 47(4):789-802 . PubMed
  13. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  14. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
RRID
AB_2563014 (BioLegend Cat. No. 138417)
AB_2563014 (BioLegend Cat. No. 138418)
Disclaimer

This product may be used for research purposes only.  It is not licensed for resale and may only be used by the buyer.  This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service.  If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California.  The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Antigen Details

Structure
KLRG1 is an inhibitory lectin-like type II transmembrane receptor containing a cytoplasmic motif similar to ITIM, expressed mainly as a homodimeric molecule consisting of two N-glycosylated subunits of approximately 30-38 kD.
Distribution

Subsets of NK cells, lymphokine-activated killer (LAK) cells and subsets of CD8+ and CD4+ cells.

Function
Regulates NK cell proliferation, maturation, and homeostasis. Inhibits immune responses by regulating the effector function and the developmental processes of NK and T cells.
Ligand/Receptor
E-cadherin
Cell Type
NK cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Antigen References

1. Grundemann C, et al. 2006. J. Immunol. 176:1311.
2. Blaser C, et al. 1998. J. Immunol. 161:6451.
3. Huntington ND, et al. 2007. J. Immunol. 178:4764.
4. Voehringer D, et al. 2001. J. Immunol. 167:4834.

Gene ID
50928 View all products for this Gene ID
UniProt
View information about KLRG1 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 10/18/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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