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PerCP/Cyanine5.5 anti-mouse IL-13 Antibody

Pricing & Availability
Clone
W17010B (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin 13
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
W17010B_PerCP-Cyanine5-5_IL-13_Antibody_102722
Cell Activation cocktail-stimulated (4 hours, in the presence of brefeldin A) Th2-polarized BALB/c CD4-positive T cells were surface stained with anti-mouse CD4 FITC. Cells were fixed, permeabilized, and intracellularly stained with anti-mouse IL-13 (clone W17010B) PerCP/Cyanine5.5 (left) or rat IgG1, κ PerCP/Cyanine5.5 isotype control (right).
  • W17010B_PerCP-Cyanine5-5_IL-13_Antibody_102722
    Cell Activation cocktail-stimulated (4 hours, in the presence of brefeldin A) Th2-polarized BALB/c CD4-positive T cells were surface stained with anti-mouse CD4 FITC. Cells were fixed, permeabilized, and intracellularly stained with anti-mouse IL-13 (clone W17010B) PerCP/Cyanine5.5 (left) or rat IgG1, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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159409 25 µg $123
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159410 100 µg $315
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Description

Mouse IL-13 was initially cloned from cDNA libraries of activated T cells and was designated as P600. IL-13 is an immunoregulatory cytokine secreted predominantly by activated Th2 cells, and it is a key mediator in the pathogenesis of allergic inflammation. IL-13 shares many functional properties with IL-4, and they share a common receptor subunit, the alpha subunit of the IL-4 receptor (IL-4Ra). IL-13 mediates its effects by interacting with a complex receptor system comprised of IL-4Ra and two IL-13 binding proteins, IL-13Ralpha1 and IL-13Ralpha2. Ligation of the IL-13 receptor complex results in signaling via the insulin receptor substrate (IRS)-1 and 2 and STAT-6 pathways. IL-13 has been implicated in inflammatory responses, such as airway hypersensitivity and mucus hypersecretion, inflammatory bowel disease, and parasitic nematode expulsion.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse IL-13
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

 

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
RRID
AB_2927973 (BioLegend Cat. No. 159409)
AB_2927973 (BioLegend Cat. No. 159410)

Antigen Details

Structure
Cytokine
Distribution

Th2 cells, basophils, mast cells, activated eosinophils and NK cells

Function
IL-13 modulates mouse monocytes/macrophages and B cells. IL-13 induces an increase of MHC class II expression in mouse monocytes/macrophages, and direct inhibition of inflammatory cytokines such as TNF-α and IL-1β.
Ligand/Receptor
IL-4Rα, IL-13Rα1, and IL-13Rα2
Cell Type
Basophils, Eosinophils, Mast cells, NK cells, Th2
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Brown KD, et al. 1989. J Immunol. 142:679-87.
  2. McKenzie AN, et al. 1993. Proc Natl Acad Sci USA. 90:3735-9.
  3. McKenzie GJ, et al. 1999. J Exp Med. 189:1565-72.
Gene ID
16163 View all products for this Gene ID
UniProt
View information about IL-13 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 10/27/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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