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PerCP/Cyanine5.5 anti-mouse Ly-6A/E (Sca-1) Antibody

Pricing & Availability
Clone
D7 (See other available formats)
Regulatory Status
RUO
Other Names
Sca-1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
D7_PerCPCyanine55_Ly-6A-E_Antibody_091919.png
C57BL/6 mouse bone marrow stained with Lineage FITC, CD117 (c-Kit) APC, and Ly-6A/E (Sca-1) (clone D7) PerCP/Cyanine5.5 (left) or Rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right). Cells gated on Lineage-negative population.
  • D7_PerCPCyanine55_Ly-6A-E_Antibody_091919.png
    C57BL/6 mouse bone marrow stained with Lineage FITC, CD117 (c-Kit) APC, and Ly-6A/E (Sca-1) (clone D7) PerCP/Cyanine5.5 (left) or Rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right). Cells gated on Lineage-negative population.
Compare all formats See PerCP/Cyanine5.5 spectral data
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108123 25 µg $118
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108124 100 µg $323
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Description

Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent mouse T-cell line (CTL-L)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, in vitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13 and Bouin-fixed, paraffin-embedded samples9.

The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14 

Application References

(PubMed link indicates BioLegend citation)
  1. Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP)
  2. Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB)
  3. Codias EK, et al. 1990. J. Immunol. 144:2197.
  4. Malek TR, et al. 1986. J. Exp. Med. 164:709.
  5. Codias EK, et al. 1990. J. Immunol. 145:1407.
  6. Ivanov V, et al. 1994. J. Immunol. 153:2394.
  7. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  8. Fleming T, et al. 1994. J. Immunol. 153:1955.
  9. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC)
  10. Umland O, et al. 2007. J. Immunol. 178:4147.
  11. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed
  12. Pronk CJ, et al. 2011. J. Exp Med. PubMed
  13. English A, et al. 2000. J. Immunol. 165:3763. (IHC)
  14. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
  15. Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed
Product Citations
  1. Tober J, et al. 2020. The Journal of Experimental Medicine. 215(2):645-659. PubMed
  2. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  3. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  4. Helm M, et al. 2022. Life (Basel). 12:. PubMed
  5. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  6. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  7. Reddy A, et al. 2020. Cell. 183:62. PubMed
  8. Taube C, et al. 2011. PLoS One. 6:e21799. PubMed
  9. Barman PK, et al. 2019. J Immunol. 202:2720. PubMed
  10. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  11. Liu W, et al. 2019. Epigenetics. 1.192361111. PubMed
  12. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  13. Zheng X, et al. 2015. Cell Rep. 13:1505-1518. PubMed
  14. de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
  15. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  16. Nenasheva T, et al. 2017. PLoS One. 12(6):e0178983. PubMed
  17. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  18. van den Berk P, et al. 2020. Cell Rep. 33:108533. PubMed
  19. DeVilbiss AW, et al. 2021. eLife. 10:00. PubMed
  20. Gago-Lopez N, et al. 2019. EMBO Mol Med. 11:e10697. PubMed
  21. Pascual R, et al. 2020. Sci Adv. 6:eaax3868. PubMed
  22. Tu Z, et al. 2022. Cell Rep. 40:111072. PubMed
  23. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  24. Gu Z, et al. 2021. Nat Genet. 53:672. PubMed
  25. Cohen I et al. 2018. Cell stem cell. 22(5):726-739 . PubMed
RRID
AB_893615 (BioLegend Cat. No. 108123)
AB_893615 (BioLegend Cat. No. 108124)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution

Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells

Function
Regulates B and T cell responses
Cell Type
B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.

Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A/E on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 4    Revision Date: 09/19/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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