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PerCP/Cyanine5.5 anti-RPS6 Phospho (Ser244) Antibody

Pricing & Availability
Clone
A18024A (See other available formats)
Regulatory Status
RUO
Other Names
Ribosomal Protein S6, 40S Ribosomal Protein S6, Phosphoprotein NP33, Small Ribosomal Subunit Protein ES6
Isotype
Mouse IgG1, κ
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Product Citations
publications
A18024A_PerCPCy5_RPS6-Phospho_Antibody_033121
Human peripheral blood lymphocytes were stimulated with (filled histogram) or without (open histogram) Cell Activation cocktail (without Brefeldin A) (Cat. No. 423302) for 10 minutes, fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer (Cat No. 425401), and intracellularly stained with anti-RPS6 Phospho (Ser244) (clone A18024A) PerCP/Cyanine5.5.
  • A18024A_PerCPCy5_RPS6-Phospho_Antibody_033121
    Human peripheral blood lymphocytes were stimulated with (filled histogram) or without (open histogram) Cell Activation cocktail (without Brefeldin A) (Cat. No. 423302) for 10 minutes, fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer (Cat No. 425401), and intracellularly stained with anti-RPS6 Phospho (Ser244) (clone A18024A) PerCP/Cyanine5.5.
Compare all formats See PerCP/Cyanine5.5 spectral data
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935709 25 tests $176
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935710 100 tests $411
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Description

Ribosomal protein S6 (RPS6) is a key component of the small 40S ribosomal subunit and is the major substrate of protein kinases in eukaryotic ribosomes. In response to various cellular stimuli such as mitogenic stimulation, insulin, and increased nutrient availability, upstream kinases such as RSK and p70 kinases phosphorylate RPS6 at multiple serine sites. These modifications facilitate the recruitment of the 7-methylguanasine cap complex, thereby promoting the assembly of the translational pre-initiation complex and increased cellular protein synthesis capacity. RPS6 has been shown to be hyperphosphorylated in certain cancers, and phosphorylation is a critical determinant of pancreatic β–cell size and systemic glucose homeostasis function in diabetic mouse models.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to human RPS6 phosphorylated at serine 244
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. 

Excitation Laser
Blue Laser (488 nm)
Application Notes

BioLegend offers two clones against this target, A18024A and A18024B:

For western blotting, A18024B displayed a higher affinity for RPS6 Phospho (Ser244) compared to A18024A. Both clones exhibit mouse reactivity.

For ICC, A18024B displayed a moderately higher affinity for RPS6 Phospho (Ser244) compared to A18024A. Both clones are mouse reactive for this application, and both clones are compatible with Triton X-100 and methanol permeabilization steps.

For ICFC, A18024A works in all three ICFC buffers (Cat# 425401, 421002, 424401). For ICFC, A18024A weakly stains mouse RPS6 Phospho (Ser244). A18024B is not recommended for ICFC due to high background staining.

Both clones are predicted to react with rat RPS6 when phosphorylated at serine 244 due to complete sequence homology between the immunizing sequence and the rat RPS6 ortholog.

RRID
AB_2892496 (BioLegend Cat. No. 935709)
AB_2892496 (BioLegend Cat. No. 935710)

Antigen Details

Structure
RPS6 is a 249 amino acid protein with a predicted molecular weight of 28.6 kD.
Distribution

Cytosol, nucleus, ER/ubiquitous expression

Function
Regulation of protein translation
Biology Area
Cell Biology, Cell Proliferation and Viability, Protein Synthesis, Signal Transduction
Molecular Family
Organelle Markers, Phospho-Proteins
Antigen References
  1. Jefferies HB, et al. 1997. EMBO J. 16:3693.
  2. Ruvinsky I, et al. 2005. Genes Dev. 19:2199-211.
  3. Schumacher AM, et al. 2006. Biochemistry. 45:13614.
  4. Roux PP, et al. 2007. J Biol Chem. 282:14056.
  5. Stevens C, et al. 2009. J Biol Chem. 284:334.
  6. Schlafli P, et al. 2011. FEBS J. 278:1757.
Gene ID
6194 View all products for this Gene ID
UniProt
View information about RPS6 Phospho on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 03/31/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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