PerCP/Fire™ 780 anti-mouse CD335 (NKp46) Antibody, CD335, 29A1.4

Pricing & Availability

Clone: 29A1.4 (See other available formats)
Regulatory Status: RUO
Other Names: NKp46, NCR1
Isotype: Rat IgG2a, κ
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Product Citations: publications

29A14_PERCP-FIRE-780_CD335_Antibody_102323 — C57BL/6 mouse splenocytes were stained with anti-mouse NK1.1 FITC and anti-mouse CD335 (NKp46) (clone 29A1.4) PerCP/Fire™ 780 (left) or stained with anti-mouse NK1.1 FITC only (right).

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137651

25 µg

$159

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137652

100 µg

$364

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Description

CD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs to the natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like) domains. It's expression is restricted to NK cells and a subset of NKT cells; it's not expressed in CD1d-restricted NKT cells. CD335 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: NKP46-IgG1 Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Fire™ 780 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Fire 780 has a maximum excitation of 478 nm and a maximum emission of 780 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections^1,2 and in vitro activation of NK cells¹.
RRID: AB_3083275 (BioLegend Cat. No. 137651)
AB_3083275 (BioLegend Cat. No. 137652)

Antigen Details

Structure: Single-pass type I membrane protein, 46 kD; belongs to the natural cytotoxicity receptor (NCR) family; contains 2 Ig-like (immunoglobulin-like) domains
Distribution: Mature and immature NK cells, subset of NKT cells, but not on CD1d-restricted NKT cells
Function: NK cells activation
Ligand/Receptor: Viral hemagglutinins, heparan sulfate proteoglycans
Cell Type: NK cells, NKT cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Colucci F and Cilio CM. 2010. Nat. Immunol. 125:60.
2. Caligiuri MA. 2008. Blood 112:461.
3. Colonna M. 2009. Immunity 31:15.
Gene ID: 17086 View all products for this Gene ID
UniProt: View information about CD335 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD335 Reagents Request Custom Conjugation

Description	Clone	Applications
Brilliant Violet 510™ anti-mouse CD335 (NKp46)	29A1.4	FC
Brilliant Violet 711™ anti-mouse CD335 (NKp46)	29A1.4	FC
PE anti-mouse CD335 (NKp46)	29A1.4	FC
PE/Cyanine7 anti-mouse CD335 (NKp46)	29A1.4	FC
Purified anti-mouse CD335 (NKp46)	29A1.4	FC,IHC-F
FITC anti-mouse CD335 (NKp46)	29A1.4	FC
APC anti-mouse CD335 (NKp46)	29A1.4	FC
PerCP/Cyanine5.5 anti-mouse CD335 (NKp46)	29A1.4	FC
Brilliant Violet 421™ anti-mouse CD335 (NKp46)	29A1.4	FC
Biotin anti-mouse CD335 (NKp46)	29A1.4	FC
Brilliant Violet 605™ anti-mouse CD335 (NKp46)	29A1.4	FC
Purified anti-mouse CD335 (NKp46) (Maxpar® Ready)	29A1.4	FC,CyTOF®
Alexa Fluor® 647 anti-mouse CD335 (NKp46)	29A1.4	FC
PE/Dazzle™ 594 anti-mouse CD335 (NKp46)	29A1.4	FC
APC/Fire™ 750 anti-mouse CD335 (NKp46)	29A1.4	FC
Brilliant Violet 650™ anti-mouse CD335 (NKp46)	29A1.4	FC
TotalSeq™-A0184 anti-mouse CD335 (NKp46)	29A1.4	PG
Brilliant Violet 785™ anti-mouse CD335 (NKp46)	29A1.4	FC
Ultra-LEAF™ Purified anti-mouse CD335 (NKp46)	29A1.4	FC,IHC-F
TotalSeq™-B0184 anti-mouse CD335 (NKp46)	29A1.4	PG
TotalSeq™-C0184 anti-mouse CD335 (NKp46)	29A1.4	PG
APC/Cyanine7 anti-mouse CD335 (NKp46)	29A1.4	FC
PE/Cyanine5 anti-mouse CD335 (NKp46)	29A1.4	FC
PerCP/Fire™ 806 anti-mouse CD335 (NKp46)	29A1.4	FC
PerCP/Fire™ 780 anti-mouse CD335 (NKp46)	29A1.4	FC
Spark Red™ 718 anti-mouse CD335 (NKp46) (Flexi-Fluor™)	29A1.4	FC
Spark Blue™ 574 anti-mouse CD335 (NKp46) (Flexi-Fluor™)	29A1.4	FC
Spark Blue™ 550 anti-mouse CD335 (NKp46) (Flexi-Fluor™)	29A1.4	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 10/23/2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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