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PerCP/Fire™ 780 anti-mouse F4/80 Antibody

Pricing & Availability
Clone
W20065D (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W20065D_PerCPFire780_F4-80_Antibody_1_091124
Thioglycolate-elicited BALB/c mouse peritoneal macrophages were stained with anti-mouse CD11b (clone M1/70) APC and anti-mouse F4/80 (clone W20065D) PerCP/Fire™ 780 (left) or rat IgG2b, κ PerCP/Fire™ 780 isotype control (right).
  • W20065D_PerCPFire780_F4-80_Antibody_1_091124
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages were stained with anti-mouse CD11b (clone M1/70) APC and anti-mouse F4/80 (clone W20065D) PerCP/Fire™ 780 (left) or rat IgG2b, κ PerCP/Fire™ 780 isotype control (right).
Compare all formats See PerCP/Fire™ 780 spectral data
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111705 25 µg $85
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111706 100 µg $220
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Description

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Fire™ 780 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Fire™ 780 has a maximum excitation of 478 nm and a maximum emission of 780 nm.

Excitation Laser
Blue Laser (488 nm)
RRID
AB_3662171 (BioLegend Cat. No. 111705)
AB_3662171 (BioLegend Cat. No. 111706)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References
  1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
  2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
  3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
  4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
  5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.
Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org
Go To Top Version: 1    Revision Date: 05/28/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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