- Clone
- W15121A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Protein kinase C inhibitor protein 1, KCIP-1, HEL4, YWHAD, HEL-S-3, HEL-S-93, 14-3-3-zeta
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
687802 | 100 µg | $229 |
In mammals, proteins of the 14-3-3 family include isoforms β, γ, ε, σ, ζ, τ, and η. 14-3-3 δ isoform is the phosphorylated form of 14-3-3 ζ. These highly conserved proteins form homo- or heterodimers with many transcription factors, metabolic enzymes, kinases, phosphatases, and other signaling molecules, generally through a phospho-Ser/Thr motif. The binding regulates the location, stability, interaction with other molecules, and phosphorylation status of the target proteins which suggests the important roles for these 14-3-3 family proteins on regulation of cellular functions and responses. Growing data demonstrate that the specific temporal and spatial distributions of 14-3-3 family proteins are critical for developmental processes; and in some cancers, the regulation of these proteins are different from healthy controls. 14-3-3 ζ interacts with IRS1 protein and is involved in the PI3K-Akt signaling pathway.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat, Drosophila
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Full length human 14-3-3 ζ/δ recombinant protein expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 – 2.0 µg/ml in human, mouse, and rat and 0.25 – 2.0 µg/ml in Drosophila. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
14-3-3 family members share a high homology in amino acid sequences. Hence, this clone may recognize other 14-3-3 family members with predicted MW of 27, 28kD.
Reactivity to Drosophila was only verified with the purified format. - RRID
-
AB_2617006 (BioLegend Cat. No. 687802)
Antigen Details
- Structure
- 245 amino acids with a predicted molecular weight of 27.7 kD.
- Distribution
-
Cytoplasm.
- Function
- 14-3-3 ζ/δ is an adaptor protein, binding to a large number of Ser/Thr phosphorylated proteins. It participates in several signaling pathways and regulates the activity of the binding partners.
- Interaction
- Forms a homooligomer or a heterodimer with other isoforms. Interacts with CDK16, BSPRY, WEE1, SAMSN1, MLF1, ITGB2, BCL2L11, FOXO4, NOXA1, SSH1, ARHGEF2, BAX, RAF1, TP53, ABL1, AANAT, AKT1, GAB2, TLK2, and DAPK2.
- Biology Area
- Cell Biology, Signal Transduction
- Antigen References
-
1. Funami K, et al. 2016. Mol. Immunol. 73:60.
2. Bonet R, et al. 2013. J. Mol. Biol. 425:3060.
3. Hong HY, et al. 2010. Mol. Cells 29:305.
4. Danes CG, et al. 2008. Cancer Res. 68:1760.
5. Niemantsverdriet M, et al. 2008. Oncogene. 27:1315.
6. Bialkowska K, et al. 2003. J. Biol. Chem. 278:33342. - Gene ID
- 7534 View all products for this Gene ID
- UniProt
- View information about 14-3-3 zeta/delta on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All 14-3-3 ζ/δ Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-14-3-3 ζ/δ | W15121A | WB,ICC |
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