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Purified anti-AKT Phospho (Thr308) Antibody

Pricing & Availability
Clone
Poly23A03 (See other available formats)
Regulatory Status
RUO
Other Names
Protein kinase B alpha, PRK-BA, PKB, Serine/Threonine specific kinase RAC alpha, Protein kinase Akt, RAC
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly23A03_PURE_AKT_WB_031824
Whole cell extracts (15 µg protein) from serum-starved HeLa cells untreated (Control) or treated with 50 ng/mL IGF-1 (Cat. No. 590904) for 30 minutes were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-AKT Phospho (Thr308) (clone Poly23A03) for 2 hours at room temperature (left blot). Proteins were visualized by chemiluminescence detection using HRP Donkey anti-rabbit IgG (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution (bottom blot). Equal AKT loading was confirmed by probing membranes with anti-Pan AKT (Total, right blot). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • Poly23A03_PURE_AKT_WB_031824
    Whole cell extracts (15 µg protein) from serum-starved HeLa cells untreated (Control) or treated with 50 ng/mL IGF-1 (Cat. No. 590904) for 30 minutes were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-AKT Phospho (Thr308) (clone Poly23A03) for 2 hours at room temperature (left blot). Proteins were visualized by chemiluminescence detection using HRP Donkey anti-rabbit IgG (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution (bottom blot). Equal AKT loading was confirmed by probing membranes with anti-Pan AKT (Total, right blot). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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636651 50 µL $305
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636652 200 µL $740
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Description

AKT (also known as protein kinase B alpha) is a 56 kDa serine/threonine-specific kinase containing a pleckstrin domain. AKT plays a critical role in controlling survival and apoptosis. This kinase is ubiquitously expressed and translocates to the membrane upon cell activation. It is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI 3-kinase. AKT is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473 by PDK2. Activated AKT phosphorylates a wide range of substrates including transcription factors (e.g. FOXO1), kinases (GSK-3beta, Raf-1, ASK, Chk1) and other proteins with important signaling roles (e.g. Bad, MDM2). AKT promotes cell survival by inhibiting apoptosis through phosphorylation of several targets, including Bad, forkhead transcription factor, c-Raf and caspase-9. AKT also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor. More importantly, AKT phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Synthetic peptide of human AKT phosphorylated at Thr308
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested dilution of this reagent is 1:500 - 1:2000. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested by western blot using lysates prepared from HeLa cells treated with or without IGF-1 (Cat. No. 590904).

RRID
AB_3097625 (BioLegend Cat. No. 636651)
AB_3097625 (BioLegend Cat. No. 636652)

Antigen Details

Structure
AKT is a 480 amino acid protein with a predicted molecular weight of 56 kD
Distribution

Ubiquitously expressed, translocates to the cytoplasmic side of the cellular membrane upon activation

Function
Catalytically inactive multimeric complex. AKT is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473 by PDK2.
Interaction
Interacts through pleckstrin homology domain with second messengers. Interacts with AKTIP, CDKN1B.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Cell Death
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References
  1. Jacinto E, et al. 2006. Cell. 127:125-37.
  2. Inoki K, et al. 2002. Nat Cell Biol. 4:648-57.
  3. Franke TF, et al. 1995. Cell. 81:727-36.
  4. Brunet A, et al. 1999. Cell. 96:857-68.
  5. Yang WL, et al. 2010. Cell. Cycle. 9:487-97.
Gene ID
207 View all products for this Gene ID
UniProt
View information about AKT on UniProt.org
Go To Top Version: 1    Revision Date: 03/07/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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