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Purified anti-AKT1 Antibody

Pricing & Availability
Clone
O94E10 (See other available formats)
Regulatory Status
RUO
Other Names
V-Akt Murine Thymoma Viral Oncogene Homolog 1, Protein Kinase B alpha, Rac Protein Kinase alpha, RAC-PK-alpha, PKB alpha, PRKBA, AKT, PKB, RAC, CWS6, PKB-ALPHA, RAC-ALPHA
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_O94E10_PURE_AKT1_Antibody_WB_110117
Total lysates (10µg protein) from HeLa and HeLa AKT1 CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:1000 (0.5µg/ml) purified anti- AKT1 antibody, clone O94E10 (upper). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse-IgG secondary antibody (cat#405306). Direct-Blot™ HRP anti-β-actin Antibody (Cat#643807) was used as a loading control (lower). Lane M is the Molecular weight ladder.
  • 1_O94E10_PURE_AKT1_Antibody_WB_110117
    Total lysates (10µg protein) from HeLa and HeLa AKT1 CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:1000 (0.5µg/ml) purified anti- AKT1 antibody, clone O94E10 (upper). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse-IgG secondary antibody (cat#405306). Direct-Blot™ HRP anti-β-actin Antibody (Cat#643807) was used as a loading control (lower). Lane M is the Molecular weight ladder.
  • 2_O94E10_PURE_AKT1_Antibody_WB_092915
    Western blot analysis of cell lysates from Jurkat (lane 1), HT29 (lane 2), HeLa (lane 3) and NIH3T3 (lane 4). Purified anti-AKT1 antibody (clone O94E10 ) was used and then followed by HRP anti-mouse IgG antibody. GAPDH antibody (clone poly6314) was used as a loading control.
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680302 100 µg $265
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Description

AKT (Protein kinase B, PKB) is a serine/threonine kinase that plays a key role in regulating cell proliferation, survival, and metabolism. Dysregulation of AKT can lead to diseases such as cancer, diabetes, along with cardiovascular and neurological diseases. There are three isoforms of AKT- AKT1, 2, and 3 (also known as PKBα, β, and γ). AKT are activated by growth factors and other extracellular stimuli as well as by oncogenic mutations in key upstream regulatory proteins, which include Ras, PI3-kinase subunits, and PTEN. Increasing evidence suggests that AKT1, 2, and 3 plays a unique role in breast cancer initiation and progression. AKT1 is cardioprotective to the heart by supporting its physiological growth and function. A reduced level of AKT1 in the frontal cortex has been found during post-mortem brain studies of schizophrenic patients in the populations of many countries.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant protein of human AKT1 expressed in HEK293T cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Rahimi B, et al. 2023. BMC Complement Med Ther. 23:131. PubMed
  2. Cataldi S, et al. 2021. Cells. 11:. PubMed
  3. Penas FN, et al. 2020. Front Immunol. 10:2955. PubMed
  4. Cevey ÁC, et al. 2021. Front Immunol. 12:782891. PubMed
RRID
AB_2566355 (BioLegend Cat. No. 680302)

Antigen Details

Structure
480 amino acids with a predicted molecular weight of approximately 56 kD.
Distribution

Cytoplasm, nucleus, cell membrane, phosphorylation on Tyr-176 by TNK52 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.

Function
AKT1 plays a key role in regulating cell proliferation, survival, and metabolism.
Interaction
BTBD10, KCTD20, CCDC88A, GRB10, AGAP2, AKTIP, MTCP1, TCL1A , TCL1B, CDKN1B, MAP3K5, BAD, PPP2R5B, STK3, STK4, SIRT1, SRPK2, RAF1, TRIM13, TNK2, CLK2, THEM4 , PDPK1 PA2G4, and KIF14.
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Neuroscience, Signal Transduction, Ubiquitin/Protein Degradation
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Perira L, et al. 2015. Drug Discov. Today 9:1152-8.
2. Yu H, et al. 2015. Vascul. Pharmacol. 71:57-64.
3. Cohen MM Jr. 2013. Am. J. Med. Genet. A. 12:2931-7.
4. Dillon RL, Muller WJ. 2010. Cancer Res. 11:4260-4.
5. Song G, et al. 2005. J. Cell Mol. Med. 1:59-71.
6. Hers I, et al. 2011. Cell Signal. 23:1515.

Gene ID
207 View all products for this Gene ID
UniProt
View information about AKT1 on UniProt.org
Go To Top Version: 3    Revision Date: 11/01/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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