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Purified anti-Alpha β Crystallin Antibody

Pricing & Availability
Clone
1329CT523.140.120 (See other available formats)
Regulatory Status
RUO
Other Names
Crystallin Alpha B, alpha-crystallin B chain, CRYAB, Heat Shock Protein Beta-5, HspB5, Renal Carcinoma Antigen NY-REN-27
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1329CT523-140-120_PURE_Alpha_Beta_Crystallin_Antibody_1_092221.png
Western blot of anti-Alpha β Crystallin antibody (clone 1329CT523.140.120). Total tissue lysates (15 µg total protein) from human skin (negative control, Lane 1), human brain (positive control, Lane 2), mouse brain (positive control, Lane 3), and rat brain (positive control, Lane 4) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 μg/mL (1:1,000) purified anti-Alpha β Crystallin antibody overnight at 4 °C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3,000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25,000 dilution (lower). Lane M: Molecular weight ladder.
  • 1329CT523-140-120_PURE_Alpha_Beta_Crystallin_Antibody_1_092221.png
    Western blot of anti-Alpha β Crystallin antibody (clone 1329CT523.140.120). Total tissue lysates (15 µg total protein) from human skin (negative control, Lane 1), human brain (positive control, Lane 2), mouse brain (positive control, Lane 3), and rat brain (positive control, Lane 4) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 μg/mL (1:1,000) purified anti-Alpha β Crystallin antibody overnight at 4 °C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3,000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25,000 dilution (lower). Lane M: Molecular weight ladder.
  • 1329CT523-140-120_PURE_Alpha_Beta_Crystallin_Antibody_2_092221.png
    IHC staining of purified anti-Alpha β Crystallin antibody (clone 1329CT523.140.120) on formalin-fixed paraffin-embedded human heart (A, positive control) and tonsil (B, negative control) tissues. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissues were incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 μm.
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948101 25 µg $118
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948102 100 µg $293
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Description

Alpha B Crystallin (CryAB) is a member of the small heat shock protein (HSP20) family.  Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. CryAB can be induced by heat shock, ischemia and oxidation. The protein is known to protect cells from stress damage. It may also play an important role in the Golgi reorganization during cell division. CryAB is also known to be associated with a number of neurodegenerative diseases such as Alzheimer’s disease and has been implicated in breast tumors of patients with shorter survival, suggesting that it may be a useful marker in identifying aggressive breast cancer.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial recombinant human alpha β crystallin protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.5 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone recognizes alpha β crystallin proteins at 21 kD in human, mouse, and rat brain lysates. An extra band at 15 kD is observed in the human brain lysates.

RRID
AB_2904461 (BioLegend Cat. No. 948101)
AB_2904461 (BioLegend Cat. No. 948102)

Antigen Details

Structure
Alpha β crystallin is a 175 amino acid protein with a predicted molecular weight of 21 kD.
Distribution

Ubiquitously expressed /Cytosol

Function
Has chaperone-like activity
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Erin-Mai FL, et al. 2021. J Neuroinflammation. 18:82.
  2. Zhihui Z and Georg R. 2018. Neurochem Int. 115:69-79.
  3. Raman B, et al. 2015. BioChim Biophys Acta. 1854:291-319.
  4. Julie A, et al. 2012. Int J Biochem Cell Biol. 44:1622-31.
Gene ID
1410 View all products for this Gene ID
UniProt
View information about Alpha beta Crystallin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/22/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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