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Purified anti-α-Synuclein (C-Terminal Truncated x-122) Antibody

Pricing & Availability
Clone
A15127A (See other available formats)
Regulatory Status
RUO
Other Names
Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-amyloid beta component (NAP), PARK1, NACP, PD1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A15127A_PURE_alpha-Synuclein_C-Terminal_Truncated_x-122_Antibody_1_IHC_011017
IHC staining of α-synuclein deposits with purified anti-α-Synuclein, C-Terminal Truncated antibody (clone A15127A) on formalin-fixed, paraffin-embedded Parkinson's disease brain tissue. Following antigen retrieval using 70% formic acid, the tissue was incubated with the primary antibody at 5 µg/mL overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
  • A15127A_PURE_alpha-Synuclein_C-Terminal_Truncated_x-122_Antibody_1_IHC_011017
    IHC staining of α-synuclein deposits with purified anti-α-Synuclein, C-Terminal Truncated antibody (clone A15127A) on formalin-fixed, paraffin-embedded Parkinson's disease brain tissue. Following antigen retrieval using 70% formic acid, the tissue was incubated with the primary antibody at 5 µg/mL overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
  • A15127A_PURE_alpha-Synuclein_C-Terminal_Truncated_x-122_Antibody_2_ELISA_011017
    Direct ELISA of purified anti-α-Synuclein, C-Terminal Truncated antibody (clone A15127A) binding to plate-immobilized recombinant human full-length (1-140) and C-terminally fragmented (1-122) α-synuclein. ELISA was performed by coating wells with 150 ng of each recombinant α-synuclein protein. The wells were then incubated with the primary antibody at 37°C for one hour, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
See high resolution IHC-P/ELISA data...
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848401 25 µg $89
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848402 100 µg $218
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Description

Alpha-synuclein is expressed principally in the nervous system, but it is also produced in other tissues, including the skin. In the brain, the protein is primarily neuronal, but it is also present in glia. Neuronal α-synuclein is concentrated in presynaptic nerve terminals, interacts with plasma membrane phospholipids, and is also present in nuclei and mitochondria. At least three isoforms of α-synuclein are produced through alternative splicing. The most common isoform is a 140 amino acid-long transcript. Other isoforms include, α-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-synuclein’s physiological role is poorly understood, but the protein has been implicated in regulating dopamine release and transport, synaptic vesicle clustering, and functioning as a SNARE-complex chaperone. α-synuclein fibrils are a major component of the intracellular Lewy bodies that are associated with Parkinson's disease, Lewy body dementia, and multiple system atrophy. Phosphorylation of α-synuclein at Tyr39 may modulate clearance of the protein and contribute to Parkinson's disease.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated synthetic peptide of α-synuclein with C-terminus terminating at Asn122.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Direct ELISA - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2.0 - 10 µg/ml is suggested. For Direct ELISA, a concentration range of 0.02 - 3.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2650690 (BioLegend Cat. No. 848401)
AB_2650690 (BioLegend Cat. No. 848402)

Antigen Details

Structure
α-synuclein's canonical isoform is a 140 amino acid protein with an apparent molecular mass of 14 kD.
Distribution

Tissue sources: Primarily nervous system, but lower expression in other tissues.
Distribution: Cytoskeleton, cytosol, lysosome, mitochondria, nucleus, plasma membrane, and extracellular.

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
α-Synuclein
Antigen References

1. Mor DE, et al. 2016. Neurobiol Dis. 88:66. PubMed
2. Jucker M & Walker LC. 2013. Nature. 501(7465):45. PubMed
3. Bartels T, et al. 2011. Nature. 477(7362): 107. PubMed
4. Devine MJ, et al. 2011. Mov Disord. 26:2160. PubMed
5. Mishizen-Eberz AJ et al. 2003. J Neurochem. 86:836. PubMed
6. Games D et al. 2014. J Neurosci. 34(28):9441. PubMed

Gene ID
6622 View all products for this Gene ID
UniProt
View information about alpha-Synuclein on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 01/16/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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