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Purified anti-α-Synuclein, Nitrated Antibody

Pricing & Availability
Clone
Syn514 (See other available formats)
Regulatory Status
RUO
Other Names
Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-A beta component of AD amyloid Parkinson disease (autosomal dominant, Lewy body) 4
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
Syn515_PURE_Synuclein_alpha_Nitrated_Antibody_030519
IHC staining of Syn514 on formalin-fixed paraffin-embedded human Parkinson's diseased brain tissue. After antigen retrieval using 70% formic acid for twenty minutes, the tissue was incubated with Syn514 at 1µg/ml overnight at 4°C. Biolegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB) was used for detection followed by hematoxylin and bluing solution counterstain.
  • Syn515_PURE_Synuclein_alpha_Nitrated_Antibody_030519
    IHC staining of Syn514 on formalin-fixed paraffin-embedded human Parkinson's diseased brain tissue. After antigen retrieval using 70% formic acid for twenty minutes, the tissue was incubated with Syn514 at 1µg/ml overnight at 4°C. Biolegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB) was used for detection followed by hematoxylin and bluing solution counterstain.
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838903 100 µg $323
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Description

Synucleins are small soluble proteins expressed primarily in neural tissues and in certain tumors. The family includes 3 known proteins: alpha-, beta-, and gamma-synuclein. All synucleins have in common a highly conserved alpha helical lipid binding motif with similarity to the class-A2 lipid binding domains of the exchangeable apolipoproteins. The alpha- and beta-synuclein proteins are found primarily in brain tissue, where they are seen mainly in the pre-synaptic terminals. Alpha-synuclein is believed to be a major component of Lewy bodies in Parkinson's disease. Mutations in alpha-synuclein are associated with rare familial cases of early onset Parkinson's disease (PD), Alzheimer's disease (AD) and several other neurodegenerative illnesses. Oxidative injury has been implicated in the pathogenesis of neurodegenerative disorders including AD and PD.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Syn514 was raised using oxidized alpha-synuclein (α-synuclein) and recognizes its native and nitrated/oxidized forms.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescent staining3,7.

Syn514 also shows some cross reactivity with beta- and gamma-synuclein.

Application References

(PubMed link indicates BioLegend citation)
  1. Duda JE, et al. 2002. Ann. Neurol. 52(2):205-10.
  2. Giasson BI, et al. 2000. Science 290:985.
  3. Waxman EA, Giasson BI. 2008. J. Neuropathol. Exp. Neurol. 5:402. (IF)
  4. Daher JP, et al. 2014. Proc. Natl. Acad. Sci. USA 25:9289. (IHC)
  5. Uryu K, et al. 2003. Exp. Neurol. 1:214. (IHC)
  6. Auluck PK, et al. 2002. Science 5556:865. (IHC)
  7. Mazzulli JR, et al. 2007. J. Biol. Chem. 43:31621. (IF)
RRID
AB_2616740 (BioLegend Cat. No. 838903)

Antigen Details

Structure
Alpha synuclein is 140 amino acids and has a molecular mass of 14 kD.
Distribution

Cytoplasm, nucleus, and extracellular.

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
α-Synuclein
Gene ID
6622 View all products for this Gene ID
UniProt
View information about alpha-Synuclein on UniProt.org
Go To Top Version: 0    Revision Date: 04/13/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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