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Purified anti-Ankyrin-B Antibody (Previously Covance catalog# MMS-5247)

Pricing & Availability
Clone
N105/17 (See other available formats)
Regulatory Status
RUO
Other Names
Ankyrin-2, ankyrin B, ankyrin, brain, non-erythroid ankyrin, ankyrin-2 nonerythrocytic
Previously
Covance Catalog# MMS-5247
Isotype
Mouse IgG2a
Ave. Rating
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Product Citations
publications
1_N105slash17_PURE_Ankyrin-B_Antibody_WB_121317
Western blot of anti-Ankyrin-B antibody (clone N105/17). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain membrane lysate; Lane 3: 30 µg of mouse brain membrane lysate; Lane 4: 30 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • 1_N105slash17_PURE_Ankyrin-B_Antibody_WB_121317
    Western blot of anti-Ankyrin-B antibody (clone N105/17). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain membrane lysate; Lane 3: 30 µg of mouse brain membrane lysate; Lane 4: 30 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • 2_N105slash17_PURE_Ankyrin_Antibody_Mouse_121317
    IHC staining of anti-Ankyrin-B antibody (clone N105/17) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
  • 3_N105slash17_PURE_Ankyrin_Antibody_Human_121317
    IHC staining of anti-Ankyrin-B antibody (clone N105/17) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
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821501 100 µL $165
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Description

Ankyrins are adaptor proteins that mediate the attachment of membrane proteins to the cytoskeleton. Ankyrin-B (Ankyrin-2) is highly expressed in cardiac tissue where it has an essential role in stabilizing ion transporters and channels in cardiomyocytes in the membrane. Mutations in ankyrin-B are associated with cardiac arrhythmia and ankyrin-B may be involved in human heart failure.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 203-496 (ankyrin repeats 6 to 14) of human Ankyrin-B.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5-10 µg per ml. For immunohistochemistry, a concentration range of 5-10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

WB Positive Control: Human, mouse and rat brain membrane lysates.

IHC Positive Control: Human, mouse and rat brain tissue.

Product Citations
  1. Vallverdú-Prats M, et al. 2023. Int J Mol Sci. 24: . PubMed
  2. Cerrone M, et al. 2017. Nat Commun. 0.406944444. PubMed
RRID
AB_2564851 (BioLegend Cat. No. 821501)

Antigen Details

Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Presynaptic proteins
Gene ID
287 View all products for this Gene ID
UniProt
View information about Ankyrin-B on UniProt.org
Go To Top Version: 4    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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