- Clone
- 1518CT337.123.86.269.232 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Apurinic/Apyrimidinic Endodeoxyribonuclease 1, REF1, HAP1, APEN, APE, APX, AP Endonuclease 1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1518CT33712386269232_PURE_APEX1_1_Antibody_011222 1518CT33712386269232_PURE_APEX1_1_Antibody_011222](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/1518CT33712386269232_PURE_APEX1_1_Antibody_011222-.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified anti-APEX1 (clone 1518CT337.123.86.269.232) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control at a 1:10000 dilution (lower). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker -
IHC staining of purified anti-APEX1 (clone 1518CT337.123.86.269.232) on formalin-fixed paraffin-embedded colon tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5.0 μg/mL of purified mouse IgG1 isotype control (Cat. No. 400102) (panel A) or 5.0 μg/mL purified anti-APEX1 (panel B) overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The images were captured with a 20X objective. Scale bar: 50 μm -
ICC staining of purified anti-APEX1 (clone 1518CT337.123.86.269.232) on HeLa cells. The cells were fixed and permeabilized with 100% methanol, and blocked with 5% FBS for 1 hour. The cells were then stained with 10 µg/mL of purified mouse IgG1, κ isotype control (Cat. No. 400102) (panel A) or 10 µg/mL of anti-APEX1 antibody (panel B), followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 anti-goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI, and the images were captured with a 40X objective. Scale bar: 50 μm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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605051 | 25 µg | $118 | ||||
605052 | 100 µg | $293 |
Apurinic-apyridimic endonuclease-1, or APEX1, is a multifunctional enzyme required for the repair of DNA damaged by both endogenous and exogenous agents. It also exerts oncogenic function as a redox signaling protein by reductively stimulating the DNA-binding activity of transcription factors that drive cancer progression, including c-Jun, AP-1, NF-κB, p53, and HIF1α. APEX1 expression level and subcellular localization are positively correlated with cancer progression, and elevated APEX1 expression may also confer resistance to DNA-damaging chemotherapeutic agents.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full-length recombinant human APEX1 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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WB - Quality tested
IHC-P, ICC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration of 5.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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When using this clone for ICC, we recommend using PFA + methanol or methanol only fix-perm conditions. We do not recommend using PFA + Triton X-100 due to non-specific staining.
When testing this clone for ICC, this antibody produced filamentous, extranuclear staining in some cells. This pattern was also observed with a control antibody generated using a different APEX1 immunogen, consistent with this staining being APEX1 dependent. - RRID
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AB_2910484 (BioLegend Cat. No. 605051)
AB_2910484 (BioLegend Cat. No. 605052)
Antigen Details
- Structure
- APEX1 is a 318 amino acid protein with a predicted molecular weight of ~36 kD.
- Distribution
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Nucleus/Ubiquitously expressed
- Function
- DNA repair enzyme
- Biology Area
- Cell Biology, DNA Repair/Replication
- Molecular Family
- Enzymes and Regulators
- Antigen References
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- Fishel ML, et al. 2008. DNA Repair. 7:177-86.
- Kim MH, et al. 2013. J Clin Invest. 123:3211-3230.
- Thakur, et al. 2014. Exp Mol Med. 46:e106.
- Gene ID
- 328 View all products for this Gene ID
- UniProt
- View information about APEX1 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All APEX1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-APEX1 | 1518CT337.123.86.269.232 | WB,ICC,IHC-P |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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