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Purified anti-Apo E, 109-116 Antibody

Pricing & Availability
Clone
A17067B (See other available formats)
Regulatory Status
RUO
Other Names
Apolipoprotein E, Alzheimer Disease 2, LDLCQ5, LPG, AD2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A17067B_Pure_APOE_Antibody_1_040518
Western blot of purified anti-APO E, 109-116 antibody (clone A17067B). Lane 1: Molecular weight marker; Lane2: 50 ng of recombinant human Apo E2; Lane 3: 50 ng of recombinant human Apo E3; Lane 4: 50 ng of recombinant human Apo E4; Lane 5: 16 µg of human plasma. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • A17067B_Pure_APOE_Antibody_1_040518
    Western blot of purified anti-APO E, 109-116 antibody (clone A17067B). Lane 1: Molecular weight marker; Lane2: 50 ng of recombinant human Apo E2; Lane 3: 50 ng of recombinant human Apo E3; Lane 4: 50 ng of recombinant human Apo E4; Lane 5: 16 µg of human plasma. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • A17067B_Pure_APOE_Antibody_2_040518
    Direct ELISA of purified anti-APO E, 109-116 antibody (clone A17067B) binding to plate-immobilized recombinant human APO E2, APO E3, and APO E4. ELISA was performed by coating wells with 100 ng of APO E proteins. The wells were then incubated with the primary antibody at 37°C for 45 minutes, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
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852701 25 µg $112
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852702 100 µg $276
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Description

Apolipoprotein E (Apo E) belongs to a class of apolipoproteins that mediate the binding, internalization, and catabolism of lipoprotein particles. Apo E is produced by the liver and macrophages in peripheral tissues where it regulates cholesterol metabolism. In the central nervous system, Apo E is primarily produced by astrocytes and plays an important role in transporting cholesterol to neurons via Apo E receptors. Apo E has three alleles (ApoE2, ApoE3 and ApoE4) that differ from each other by only one or two amino acids at positions 112 and 158. ApoE 4 is one of the largest known genetic risk factors for late-onset Alzheimer's disease. Furthermore, Apo E is associated with cardiovascular disease, Lipoprotein Glomerulopathy and Sea-Blue Histiocyte Disease.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Direct ELISA - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. For Direct ELISA applications, a concentration range of 0.004 - 0.4 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Mahley RW. 2016. Arterioscler Thromb Vasc Biol. 36: 1305-1315.
  2. Riedel BC, et al. 2016. J Steroid Biochem Mol Biol. 160:134-47.
  3. Tai LM, et al. 2016. Acta Neuropathol. 131(5):709-23.
  4. Kim J, et al. 2014. Mol. Cells. 37:767
  5. Holtzman DM, et al. 2012. Cold Spring Harb. Perspect. Med. 2:a006312
RRID
AB_2728597 (BioLegend Cat. No. 852701)
AB_2728597 (BioLegend Cat. No. 852702)

Antigen Details

Structure
Human APO E is a 299 amino acid protein with a molecular mass of 32 kD, and exists as 3 major isoforms, APO E2, APO E3, and APO E4.
Distribution

Tissue sources: Ubiquitously expressed. Highest levels in liver, brain, spleen, adrenal gland, and kidney.

Cellular distribution: Plasma membrane, nucleus, endoplasmic reticulum, golgi apparatus, endosome, and extracellular.

Function
Apo E is involved in lipoprotein metabolism as well as the transport of fat-soluble vitamins, and cholesterol. Apo E also forms complexes with amyloid beta peptides leading to its uptake and degradation by microglia.
Interaction
Tau, amyloid beta
Ligand/Receptor
LDL receptor, SORL1
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Apolipoproteins
Antigen References
  1. Mahley RW. 2016. Arterioscler Thromb Vasc Biol. 36: 1305-1315.
  2. Riedel BC, et al. 2016. J Steroid Biochem Mol Biol. 160:134-47.
  3. Tai LM, et al. 2016. Acta Neuropathol. 131(5):709-23.
  4. Kim J, et al. 2014. Mol. Cells. 37:767
  5. Holtzman DM, et al. 2012. Cold Spring Harb. Perspect. Med. 2:a006312
Gene ID
348 View all products for this Gene ID
UniProt
View information about Apo E 109-116 on UniProt.org
Go To Top Version: 0    Revision Date: 04/05/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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