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Purified anti-Apo E, 109-116 Antibody

Pricing & Availability
Clone
A17067B (See other available formats)
Regulatory Status
RUO
Other Names
Apolipoprotein E, Alzheimer Disease 2, LDLCQ5, LPG, AD2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A17067B_Pure_APOE_Antibody_1_040518
Western blot of purified anti-APO E, 109-116 antibody (clone A17067B). Lane 1: Molecular weight marker; Lane2: 50 ng of recombinant human Apo E2; Lane 3: 50 ng of recombinant human Apo E3; Lane 4: 50 ng of recombinant human Apo E4; Lane 5: 16 µg of human plasma. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • A17067B_Pure_APOE_Antibody_1_040518
    Western blot of purified anti-APO E, 109-116 antibody (clone A17067B). Lane 1: Molecular weight marker; Lane2: 50 ng of recombinant human Apo E2; Lane 3: 50 ng of recombinant human Apo E3; Lane 4: 50 ng of recombinant human Apo E4; Lane 5: 16 µg of human plasma. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • A17067B_Pure_APOE_Antibody_2_040518
    Direct ELISA of purified anti-APO E, 109-116 antibody (clone A17067B) binding to plate-immobilized recombinant human APO E2, APO E3, and APO E4. ELISA was performed by coating wells with 100 ng of APO E proteins. The wells were then incubated with the primary antibody at 37°C for 45 minutes, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
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852701 25 µg $112
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852702 100 µg $276
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Description

Apolipoprotein E (Apo E) belongs to a class of apolipoproteins that mediate the binding, internalization, and catabolism of lipoprotein particles. Apo E is produced by the liver and macrophages in peripheral tissues where it regulates cholesterol metabolism. In the central nervous system, Apo E is primarily produced by astrocytes and plays an important role in transporting cholesterol to neurons via Apo E receptors. Apo E has three alleles (ApoE2, ApoE3 and ApoE4) that differ from each other by only one or two amino acids at positions 112 and 158. ApoE 4 is one of the largest known genetic risk factors for late-onset Alzheimer's disease. Furthermore, Apo E is associated with cardiovascular disease, Lipoprotein Glomerulopathy and Sea-Blue Histiocyte Disease.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Direct ELISA - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. For Direct ELISA applications, a concentration range of 0.004 - 0.4 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Mahley RW. 2016. Arterioscler Thromb Vasc Biol. 36: 1305-1315.
  2. Riedel BC, et al. 2016. J Steroid Biochem Mol Biol. 160:134-47.
  3. Tai LM, et al. 2016. Acta Neuropathol. 131(5):709-23.
  4. Kim J, et al. 2014. Mol. Cells. 37:767
  5. Holtzman DM, et al. 2012. Cold Spring Harb. Perspect. Med. 2:a006312
RRID
AB_2728597 (BioLegend Cat. No. 852701)
AB_2728597 (BioLegend Cat. No. 852702)

Antigen Details

Structure
Human APO E is a 299 amino acid protein with a molecular mass of 32 kD, and exists as 3 major isoforms, APO E2, APO E3, and APO E4.
Distribution

Tissue sources: Ubiquitously expressed. Highest levels in liver, brain, spleen, adrenal gland, and kidney.

Cellular distribution: Plasma membrane, nucleus, endoplasmic reticulum, golgi apparatus, endosome, and extracellular.

Function
Apo E is involved in lipoprotein metabolism as well as the transport of fat-soluble vitamins, and cholesterol. Apo E also forms complexes with amyloid beta peptides leading to its uptake and degradation by microglia.
Interaction
Tau, amyloid beta
Ligand/Receptor
LDL receptor, SORL1
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Apolipoproteins
Antigen References
  1. Mahley RW. 2016. Arterioscler Thromb Vasc Biol. 36: 1305-1315.
  2. Riedel BC, et al. 2016. J Steroid Biochem Mol Biol. 160:134-47.
  3. Tai LM, et al. 2016. Acta Neuropathol. 131(5):709-23.
  4. Kim J, et al. 2014. Mol. Cells. 37:767
  5. Holtzman DM, et al. 2012. Cold Spring Harb. Perspect. Med. 2:a006312
Gene ID
348 View all products for this Gene ID
UniProt
View information about Apo E 109-116 on UniProt.org

Related FAQs

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Go To Top Version: 0    Revision Date: 04/05/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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