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Purified anti-Apo E, 154-162 Antibody

Pricing & Availability
Clone
A17065A (See other available formats)
Regulatory Status
RUO
Other Names
Apolipoprotein E, APO-E, Alzheimer Disease 2, LDLCQ5, APOE, LPG, AD2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A17065A_Pure_ApoE154-163_Antibody_1_041018
Western blot of purified anti-Apo E, 154-162 antibody (clone A17065A). Lane 1: Molecular weight marker; Lane 2: 50 ng of recombinant human Apo E2; Lane 3: 50 ng of recombinant human Apo E3; Lane 4: 50 ng of recombinant human Apo E4; Lane 5: 35 µg of human plasma; Lane 6: 70 µg of human plasma; Lane 7: 140 µg of human plasma. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • A17065A_Pure_ApoE154-163_Antibody_1_041018
    Western blot of purified anti-Apo E, 154-162 antibody (clone A17065A). Lane 1: Molecular weight marker; Lane 2: 50 ng of recombinant human Apo E2; Lane 3: 50 ng of recombinant human Apo E3; Lane 4: 50 ng of recombinant human Apo E4; Lane 5: 35 µg of human plasma; Lane 6: 70 µg of human plasma; Lane 7: 140 µg of human plasma. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • A17065A_Pure_ApoE154-163_Antibody_2_041018
    Direct ELISA of purified anti-Apo E, 154-162 antibody (clone A17065A) binding to plate-immobilized recombinant human Apo E2, Apo E3, and Apo E4. ELISA was performed by coating wells with 100 ng of Apo E proteins. The wells were then incubated with the primary antibody at 37°C for 45 minutes, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
  • A17065A_Pure_ApoE154-163_Antibody_3_022820
    IHC staining of purified anti-Apo E, 154-162 antibody (clone A17065A) on formalin-fixed paraffin-embedded Alzheimer’s disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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852801 25 µg $112
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852802 100 µg $276
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Description

Apolipoprotein E (Apo E) belongs to a class of apolipoproteins that mediate the binding, internalization, and catabolism of lipoprotein particles. Apo E is produced by the liver and macrophages in peripheral tissues where it regulates cholesterol metabolism. In the central nervous system, Apo E is primarily produced by astrocytes and plays an important role in transporting cholesterol to neurons via Apo E receptors. Apo E has three alleles (Apo E2, Apo E3 and Apo E4) that differ from each other by only one or two amino acids at positions 112 and 158. Apo E4 is one of the largest known genetic risk factors for late-onset Alzheimer's disease. Furthermore, Apo E is associated with cardiovascular disease, Lipoprotein Glomerulopathy and Sea-Blue Histiocyte Disease.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH conjugated Apo E peptide encompassing amino acids 154-162.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Direct ELISA, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per mL. For Direct ELISA applications, a concentration range of 0.004 - 0.4 µg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody cross-reacts with Apo E2, Apo E3, and Apo E4.

RRID
AB_2728599 (BioLegend Cat. No. 852801)
AB_2728599 (BioLegend Cat. No. 852802)

Antigen Details

Structure
Human Apo E is a 299 amino acid protein with a molecular mass of 32 kD, and exists as 3 major alleles, Apo E2, Apo E3, and Apo E4.
Distribution

Tissue sources: Ubiquitously expressed. Highest levels in liver, brain, spleen, adrenal gland, and kidney.
Cellular distribution: Plasma membrane, nucleus, endoplasmic reticulum, golgi apparatus, endosome, and extracellular.

Function
Apo E is involved in lipoprotein metabolism as well as the transport of fat-soluble vitamins, and cholesterol. Apo E also forms complexes with amyloid beta peptides leading to its uptake and degradation by microglia.
Interaction
Tau, amyloid beta
Ligand/Receptor
LDL receptor, SORL1.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Apolipoproteins
Antigen References
  1. Mahley RW. 2016. Arterioscler Thromb Vasc Biol. 36: 1305-1315.
  2. Riedel BC, et al. 2016 J Steroid Biochem Mol Biol. 160:134-47.
  3. Tai LM, et al. 2016 Acta Neuropathol. 131(5):709-23.
  4. Kim J, et al. 2014. Mol. Cells. 37:767
  5. Holtzman DM, et al. 2012. Cold Spring Harb. Perspect. Med. 2:a006312
Gene ID
348 View all products for this Gene ID
UniProt
View information about Apo E 154-162 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 02/28/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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