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Purified anti-Apo E Antibody (Previously Covance catalog# SIG-39750)

Pricing & Availability
Clone
E6D7 (See other available formats)
Regulatory Status
RUO
Other Names
Apolipoprotein E, apolipoprotein E3, AD2, LDLCQ5, LPG, ApoE
Previously
Signet Catalog# 9750-02
Signet Catalog# 9750-05
Signet Catalog# 9750-10
Covance Catalog# SIG-39750
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_E6D7_PUREAF_ApoE_Proteins_WB_113018
Western blot of purified anti-Apo E antibody (clone E6D7). Lane 1: Molecular weight marker; Lane 2: 0.05 µg of APO E2 recombinant protein; Lane 3: 0.05 µg of APO E3 recombinant protein; Lane 4: Lane 3: 0.05 µg of APO E4 recombinant protein. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A_E6D7_PUREAF_ApoE_Proteins_WB_113018
    Western blot of purified anti-Apo E antibody (clone E6D7). Lane 1: Molecular weight marker; Lane 2: 0.05 µg of APO E2 recombinant protein; Lane 3: 0.05 µg of APO E3 recombinant protein; Lane 4: Lane 3: 0.05 µg of APO E4 recombinant protein. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • B_E6D7_PUREAF_ApoE_Plasma_WB_113018
    Western blot of purified anti-Apo E antibody (clone E6D7). Lane 1: Molecular weight marker; Lane 2: 40 µg of human plasma. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Check Availability Save
803401 200 µL $561
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803403 1 mL $1748
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Description

Apo-E is a class of apolipoprotein primarily synthesized in the liver, but is also found in other tissues such as brain, kidneys, and spleen. Apo-E is a protein component of serum chylomicron and lipoprotein particles, playing essential roles in cholesterol transportation, homeostasis, and metabolism. The expression of Apo-E is induced by cholesterol-rich diets and hyperlipoproteinemia. In the central nervous system, Apo-E is produced by astrocytes and microglia. The Apo-E-containing lipoprotein particles play important functions in brain injury repair, neurogenesis, and synaptic plasticity. Apo-E has been found to be involved in the regulation of amyloid-β aggregation and clearance. Genetic polymorphisms of Apo-E are associated with neuron degenerative disorders including Alzheimer's disease.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against a synthetic peptide corresponding to amino acids surrounding the polymorphic amino acid position 158 of Apo-E.
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB).

Clone E6D7 recognizes the E2, E3, and E4 isoforms of apolipoprotein E.

Application References

(PubMed link indicates BioLegend citation)
  1. Munoz DG, Feldman H. 2000. CMAJ. 162(1):65-72.
  2. Hagberg JM, et al. 2000. Physiol Genomics 4(2):101-108.
  3. Saunders AM. 2000. J. Neuropathol. Exp. Neurol. 59(9):751-8.
  4. Golabek AA, et al. 2000. Biophys. J. 79(2):1008-15.
  5. Zunarelli E, et al. 2000. Breast Cancer Res. Treat. 63(3):193-8.
  6. Zunarelli E, et al. 2000. Clin. Neuropathol. 19(1):1-6.
  7. Tomiyama T, et al. 1999. Cell Mol. Life Sci. 56(3-4):268-79.
  8. Golabek AA., et al. 1999. J. Biol. Chem. 271(18):10602-6.
RRID
AB_2564663 (BioLegend Cat. No. 803401)
AB_2564663 (BioLegend Cat. No. 803403)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family
Apolipoproteins
Gene ID
348 View all products for this Gene ID
UniProt
View information about Apo E on UniProt.org
Go To Top Version: 5    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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