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Purified anti-Apo E4 Antibody (Previously Covance catalog# SIG-39754)

Pricing & Availability
Clone
9D11 (See other available formats)
Regulatory Status
RUO
Other Names
Apolipoprotein E, apoloprotein E4
Previously
Covance Catalog# SIG-39754
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
9D11_Purified_Apo-E4_Antibody_1_061819.png
Capture ELISA of purified anti-Apo E4 antibody (clone 9D11) binding to recombinant human Apo E4 and Apo E3 proteins. ELISA was performed by coating wells with 150 ng of purified anti-Apo E4 antibody (clone 9D11). The wells were then incubated at 37°C for 2 h with serially diluted (0.016 µg/mL to 1 µg/ml) recombinant proteins, followed by incubation with 1.5 µg/mL of biotin anti-Apo E (clone D6E10) as the detection antibody at 37°C for 45 minutes. HRP Streptavidin (Cat. No. 405210) was used as secondary antibody and TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
  • 9D11_Purified_Apo-E4_Antibody_1_061819.png
    Capture ELISA of purified anti-Apo E4 antibody (clone 9D11) binding to recombinant human Apo E4 and Apo E3 proteins. ELISA was performed by coating wells with 150 ng of purified anti-Apo E4 antibody (clone 9D11). The wells were then incubated at 37°C for 2 h with serially diluted (0.016 µg/mL to 1 µg/ml) recombinant proteins, followed by incubation with 1.5 µg/mL of biotin anti-Apo E (clone D6E10) as the detection antibody at 37°C for 45 minutes. HRP Streptavidin (Cat. No. 405210) was used as secondary antibody and TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
  • 9D11_Purified_Apo-E4_Antibody_2_061819.png
    Capture ELISA of purified anti-Apo E4 antibody (clone 9D11) binding to human plasma. ELISA was performed by coating wells with 150 ng of purified anti-Apo E4 antibody (clone 9D11). The wells were then incubated at 37°C for 2 h with serially diluted (0.078 µg/mL to 5 µg/ml) human plasma, followed by incubation with 1.5 µg/mL of biotin anti-Apo E (clone D6E10) as the detection antibody at 37°C for 45 minutes. HRP Streptavidin (Cat. No. 405210) was used as secondary antibody and TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
Cat # Size Price Quantity Check Availability Save
813701 200 µL $398
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813702 1 mL $1647
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Description

Apolipoprotein E (ApoE) belongs to a class of apolipoproteins that binds to lipids to form lipoproteins. ApoE is produced by the liver and macrophages in peripheral tissues where it regulates cholesterol metabolism. In the central nervous system, ApoE is primarily produced by astrocytes and plays an important role in transporting cholesterol to neurons via ApoE receptors. ApoE has three major isoforms corresponding to three alleles (ApoE2, ApoE3 and ApoE4) that differ from each other by only one or two amino acids at positions 112 and 158. ApoE4 is one of the largest known genetic risk factors for late-onset Alzheimer's disease. Further, ApoE4 has been implicated in several other diseases such as atherosclerosis, impaired cognitive function and multiple sclerosis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

ELISA Capture - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 3 - 5 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is specific for the E4 isoform of apolipoprotein E.

Product Citations
  1. Jablonski AM, et al. 2021. Sci Rep. 11:3438. PubMed
RRID
AB_2564774 (BioLegend Cat. No. 813701)
AB_2564774 (BioLegend Cat. No. 813702)

Antigen Details

Structure
Predicted MW = 34 kD
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Gene ID
348 View all products for this Gene ID
UniProt
View information about Apo E4 on UniProt.org
Go To Top Version: 4    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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