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Purified anti-Arl13b Antibody

Pricing & Availability
Clone
N295B/66 (See other available formats)
Regulatory Status
RUO
Other Names
ADP-ribosylation factor-like protein 13B, ADP-ribosylation factor-like protein 2-like 1, ARL2-like protein 1 and Arl2l1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
N295B-66_Purified_Arl13b_1_112018
Western blot of purified anti-Arl13b antibody (clone N295B/66). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain lysate; Lane 3: 20 µg of rat brain lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti- mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N295B-66_Purified_Arl13b_1_112018
    Western blot of purified anti-Arl13b antibody (clone N295B/66). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain lysate; Lane 3: 20 µg of rat brain lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti- mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N295B-66_Purified_Arl13b_2_112018
    IHC staining of purified anti-Arl13b antibody (clone N295B/66) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • N295B-66_Purified_Arl13b_3_112018
    IHC staining of purified anti-Arl13b antibody (clone N295B/66) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
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857601 25 µg $83
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857602 100 µg $165
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Description

The ADP-ribosylation factor (Arf)-like small GTPase Arl13b is a small GTPase mainly found in primary cilia of neuroeptithelial cells, radial glial cells and fibroblast. The Arl13b null mice display multiple structural defects in the neural tube, limbs, and eyes. Loss of Arl13b function is associated with impaired sonic hedgehog signaling, disrupted cilia polarity and scaffold in radial progenitor cells. Three mutations in human Arl13b gene have been identified in Joubert Syndrom (JS) patients, two of which are located in the GTP-binding sensitive switch region, known as switch 2 region. Consistent with the mutant phenotype in the mouse null allele, Joubert syndrome patients are characterized with neural, ocular and renal defects, and mental retardation. Multiple studies have suggested that the role of Arl13b in ciliogenesis is mediated by the sonic hedgehog signaling.

Recently, Arl13b was also shown to mediate endocytic trafficking of CD1a, an MHC-class 1 like antigen presenting molecule which presents lipids rather proteins. Loss of Arl13b causes the clustering of early endosomes and accumulation of endocytic cargos. In this process, Arl13 colocalizes with 2 other endocytic markers, Arf2 and Rab22a, and may interact with actin cytoskeleton.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein corresponding to amino acids 208-427 (C-terminus) of mouse Arl13b.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2801216 (BioLegend Cat. No. 857601)
AB_2801216 (BioLegend Cat. No. 857602)

Antigen Details

Structure
Murine Arl13b is a 427 amino acid protein with a molecular mass of 48 kD.
Distribution

Tissue distribution: Neuroepithelial cells, developing radial glial cells, and fibroblasts.
Cellular distribution: Localized in primary cilium, usually at apical cell soma domains of cells.

Function
Ciliogenesis in multiple organs; regulation of radial glial cell polarity and scaffold; control of microtube-based, ciliary axoneme structure; endocytic recycling and trafficking.
Interaction
Arl13b interacts with tubulin, actin cytoskeleton, and SUMO-conjugating enzyme UBC-9.
Ligand/Receptor
Sonic hedgehog signaling ligand like Smoothened and Patched; ciliary sensory receptors
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Postsynaptic proteins
Antigen References
  1. Barral D.C. et al. 2012. Proc Natl Acad Sci U S A. 109(52), 21354-9.
  2. Caspary, T.et al. 2007.Developmental Cell. 12, 767–778.
  3. Higginbotham, H. et al. 2013. Nat. Neurosci. 16(8):1000-7.
  4. Ivanova, A.A. et al. 2017. J Biol Chem. 292(26), 11091–11108.
  5. Li Y. et al. 2012. J. Cell Biol. 199(4), 589-98.
Gene ID
68146 View all products for this Gene ID
UniProt
View information about Arl13b on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/21/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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