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Purified anti-Artemis Antibody

Pricing & Availability
Clone
15E7C90 (See other available formats)
Regulatory Status
RUO
Other Names
Protein artemis, DNA cross-link repair 1C protein (DCLRE1C), ASCID, SCIDA, SNM1 homolog C (SNM1C)
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_15E7C90_PURE_Artemis_Antibody_100716
Total cell lysates (15 µg total protein) from PC-3, A549 and HaCat cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1 µg/mL (1:500 dilution) of Purified anti-Artemis Antibody, clone 15E7C90, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Cell lysates were loaded in order of increasing predicted DCLREC1 mRNA expression.
  • 1_15E7C90_PURE_Artemis_Antibody_100716
    Total cell lysates (15 µg total protein) from PC-3, A549 and HaCat cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1 µg/mL (1:500 dilution) of Purified anti-Artemis Antibody, clone 15E7C90, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Cell lysates were loaded in order of increasing predicted DCLREC1 mRNA expression.
  • 2_15E7C90_PURE_Artemis_Antibody_100716
    Total cell lysate from human HeLa cells (lane 1) and mouse RAW264.7 cells (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-Artemis (clone 15E7C90) antibody. Proteins were visualized using an HRP goat anti-mouse IgG antibody and chemiluminescence detection. Purified anti-β-actin (clone Poly6221) antibody was used as a loading control.
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691602 100 µg $253
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Description

Artemis, also known as DCLRE1C, is a nuclear protein belonging to the metallo-β-lactamase superfamily. Artemis has been found to be required for the assembling of B cell antibody genes and T cell receptor genes from individual V, D, and J segments. During V(D)J recombination, Artemis forms a complex with DNA-dependent protein kinase (DNA-‘PK) and cuts the DNA with hairpin structure. Deficiency in Artemis results in blocking of V(D)J recombination and failure to produce mature B and T cells, which is known as the condition of severe combined immune deficiency (SCID). Artemis is also found to be involved in the termini processing step of double-strand break (DSB) repair. Artemis-deficient cells are more sensitive to DSB induced by ionizing radiation or chemical agents such as bleomycin and neocarzinostatin.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human Artemis recombinant protein (347-692 aa) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody has a similar affinity to BioLegend clone 13A9C90 for human Artemis by western blot, but does not recognize mouse Artemis

Product Citations
  1. Niewolik D, et al. 2022. Nucleic Acids Res. 50:2096. PubMed
RRID
AB_2632653 (BioLegend Cat. No. 691602)

Antigen Details

Structure
692 amino acids with a predicted molecular weight of 78.4 kD. Four isoforms produced by alternative splicing.
Distribution

Nucleus.

Function
Artemis is a nuclear protein exhibiting single-strand-specific 5'-3' exonuclease activity and endonuclease activity. It plays crucial roles in V(D)J recombination and DNA repair.
Interaction
Interacts with ATM, BRCA1, PRKDC and TP53BP1.
Biology Area
Cell Biology, DNA Repair/Replication
Antigen References

1. Chang HH, et al. 2016. Nucleic Acids Res. 44:4991.
2. Volk T, et al. 2015. Hum. Mol. Genet. 24:7361.
3. Waide EH, et al. 2015. J. Immunol. 195:3171.
4. Lee PP, et al. 2013. Clin. Immunol. 149:464.
5. Köcher S, et al. 2012. Nucleic Acids Res. 40:8336.
6. Beucher A, et al. 2009. EMBO J. 28:3413.

Gene ID
64421 View all products for this Gene ID
UniProt
View information about Artemis on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 11/30/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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