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Purified anti-ASC Antibody

Pricing & Availability
Clone
O93E9 (See other available formats)
Regulatory Status
RUO
Other Names
PYD and CARD domain-containing protein (PYCARD), Apoptosis-associated speck-like protein containing a CARD, Caspase recruitment domain protein 5, Target of methylation-induced silencing (TMS1)
Isotype
Mouse IgG1, κ
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Product Citations
publications
093E9_Purified_ASC_Antibody_WB_043015
Total cell lysate of HL-60 (lane 1, 15 µg), HT-29 (lane 2, 15 µg), and mouse thymus (lane 3, 15 µg) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-ASC (clone O93E9) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • 093E9_Purified_ASC_Antibody_WB_043015
    Total cell lysate of HL-60 (lane 1, 15 µg), HT-29 (lane 2, 15 µg), and mouse thymus (lane 3, 15 µg) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-ASC (clone O93E9) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
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676502 100 µg $311
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Description

Apoptosis-associated speck-like protein containing a CARD (ASC), also known as target of methylation-mediated silencing (TMS1), PYD, and CARD domain containing protein (PYCARD), is a 21.6 kD pro-apoptotic protein that contains a N-terminal pyrin domain (PYD) and a C-terminal caspase recruitment domain (CARD). The TMS1 gene was originally found to be aberrantly methylated and silenced in various cancer cells. Expression of ASC can be induced by pro-apoptotic/inflammatory stimuli. In normal cells, this protein is localized to the cytoplasm. However, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. During apoptosis, ASC translocates from the cytosol to the mitochondria and associates with mitochondrial Bax to trigger cytochrome c release and subsequent apoptosis. The adaptor molecule ASC mediates AIM2-dependent caspase-1 activation. Also, the AIM2/ASC complex can act as a novel caspase-8 activation platform. Recent studes has shown that ASC reduces the phosphorylation of IKKα/β phosphorylation and inhibits NF-κB activity in primary melanoma. However, in metastatic melanoma through, it has the opposite function enhancing NF-κB activity and the secretion of IL-1β.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant human PYCARD (NP_037390) produced in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Gritsenko A, et al. 2022. Eur J Immunol. 52:1141. PubMed
  2. Yan YQ, et al. 2022. Eur J Neurol. 29:3590. PubMed
  3. Santana DJ, et al. 2020. Curr Protoc Microbiol. 59:e124. PubMed
RRID
AB_2565643 (BioLegend Cat. No. 676502)

Antigen Details

Structure
Full-length ASC (fASC) contains 195 amino acids wtih a predicted molecular weight of 21.6 kD. The sequence of isoform ASC-b is missing 93-111 a.a. The sequence of isoform ASC-c is missing 26-85 a.a.
Distribution

Cytoplasm. In resting monocytes and macrophages, the nucleus is the primary localization.

Function
ASC promotes caspase-mediated apoptosis. This pro-apoptotic activity is mediated predominantly through the activation of caspase-9. ASC is an integral component of the inflammasome. Within the inflammasome, ASC links caspase-1 and NOD-like receptors (NLR), which lead to the activation of caspase-1.
Ligand/Receptor
CIAS1/PYPAF1 and PYDC1.
Cell Type
Dendritic cells
Biology Area
Angiogenesis, Cell Biology, Immunology, Innate Immunity, Neuroscience, Signal Transduction
Antigen References

1. Masumoto J, et al. 1999. J. Biol. Chem. 274:33835.
2. Conway KE, et al. 2000. Cancer Res. 60:6236.
3. Strong R, et al. 1991. Brain Res. 542:23.
4. Srinivasula SM, et al. 2002. J. Biol. Chem. 277:21119.
5. Ohtsuka T, et al. 2004. Nat. Cell Biol. 6:121.
6. Ippagunta SK, et al. 2010. J. Biol. Chem. 285:12454.
7. Liu W, et al. 2013. J. Invest Dermatol. 2:518-527.

Gene ID
29108 View all products for this Gene ID
UniProt
View information about ASC on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/10/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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