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Purified anti-ATF-2 Antibody

Pricing & Availability
Clone
W21106A (See other available formats)
Regulatory Status
RUO
Other Names
activating transcription factor 2 formerly, ATF2, cAMP-responsive element-binding protein 2, CREB2, CREBP1, CRE-BP1, cyclic AMP-dependent transcription factor ATF-2, HB16, MGC111558, TREB7
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A. W21106A_PURE_ATF_WB_102123
Whole cell extracts (15 µg total protein per lane) from the indicated cell lines (lane 1 and 2 are HeLa cells without and with ATF-2 knockdown, respectively) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-ATF-2 (clone W21106A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-Rat IgG (Cat. No. 405405) at a 1:5000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W21106A_PURE_ATF_WB_102123
    Whole cell extracts (15 µg total protein per lane) from the indicated cell lines (lane 1 and 2 are HeLa cells without and with ATF-2 knockdown, respectively) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-ATF-2 (clone W21106A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-Rat IgG (Cat. No. 405405) at a 1:5000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W21106A_PURE_ATF_ICC_102123
    HeLa cells without (panel A and B) and with (panel C and D) ATF-2 knockdown were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton-X, and then blocked with 5% FBS for 1 hour at room temperature. Cells were then intracellularly stained with 10 µg/mL of purified anti-ATF-2 (clone W21106A), followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 Goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The image was captured with a 40X objective. Scale bar: 25 µm
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630051 25 µg $205
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630052 100 µg $495
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Description

The Activating Transcription Factor 2 (ATF-2), also known as CREB2 and CREBP1, contains a Basic Leucine Zipper (bZIP) domain and a C2H2-type zinc finger domain. ATF-2 binds to both DNA response factors AP-1 and CRE and belongs to the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cytosolic tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. ATF-2 activity can be initiated by various forms of cellular stress. Several studies have shown aberrant ATF-2 activation in the development and progression of mammalian skin tumors. ATF-2 may mediate carcinogenesis induced by the mutant Ras protein and regulate the maintenance of the invasive cancer phenotype of several epithelial cell types. ATF-2 has also been shown to be phosphorylated at its C-terminus (serine 472 and 480 in mice, serine 490 and 498 in human) by ATM during double-strand breaks. Mice with mutations of these two serines are sensitive to irradiation and more prone to tumorigenesis under p53 knockout condition.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial Recombinant human ATF2 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 2.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The clone cross-reacts with mouse in western blot (WB).

For immunocytochemistry (ICC), 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 is recommended.

This product is not recommended for use in immunohistochemistry (IHC).

Additional Product Notes

This antibody has been tested in knockout/knockdown models for Western Blotting and Immunocytochemistry.

RRID
AB_3097336 (BioLegend Cat. No. 630051)
AB_3097336 (BioLegend Cat. No. 630052)

Antigen Details

Structure
ATF-2 protein is a 505 amino acid protein with a predicted molecular weight of 55 kD
Distribution

Abundant expression in the brain

Function
Transcriptional activator
Interaction
SMAD3, SMAD4, UTF1, NBN, MRE11, XPO1, KAT5, CUL3
Biology Area
Cell Biology, DNA Repair/Replication, Transcription Factors
Antigen References
  1. Abdel-Hafiz, H.A. et al. 1992. Mol Endocrinol. 6:2079-89.
  2. Gupta S, et al. 1995. Science. 267:389-93.
  3. Van Dam H, et al. 1995. EMBO J. 14:1798-811.
  4. Livingstone C, et al. 1995. EMBO J. 14:1785-97.
  5. Yamasaki T, et al. 2009. J Cell Biochem. 284:8567-81.
  6. Leslie MC, et al. 2009. J Cell Biochem. 94:25–38.
  7. Papassava P, et al. 2004. Cancer Res. 64:8573–84.
  8. Vlahopoulos SA, et al. 2008. BioEssays. 30:314–27.
  9. Bhoumik A, et al. 2005. Mol.Cell. 18:577–87.
  10. Li S, et al. 2010. Genes Cancer. 1:316–330.
Gene ID
1386 View all products for this Gene ID
UniProt
View information about ATF-2 on UniProt.org
Go To Top Version: 1    Revision Date: 10/23/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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